Biomimetic Calcium Phosphate Crystal Mineralization on Electrospun Cellulose-Based Scaffolds
Artikel i vetenskaplig tidskrift, 2011

Novel cellulose based-scaffolds were studied for their ability to nucleate bioactive calcium phosphate crystals for future bone healing applications. Cellulose-based scaffolds were produced by electrospinning cellulose acetate (CA) dissolved in a mixture of acetone/dimethylacetamide (DMAc). The resulting nonwoven CA mats containing fibrils with diameters in the range of 200 nm to 1.5 mu m were saponified by NaOH/ethanol for varying times to produce regenerated cellulose scaffolds. Biomimetic crystal growth nucleated from the fiber surface was studied as a function of surface chemistry. Regenerated cellulose scaffolds of varying treatments were soaked in simulated body fluid (SBF) solution. Scaffolds that were treated with CaCl2, a mixture of carboxymethyl cellulose (CMC) and CaCl2 and NaOH and CaCl2, were analyzed using X-ray photoelectron spectroscopy, X-ray powder diffraction, and scanning electron microscopy to understand the growth of bioactive calcium phosphate (Ca-P) crystals as a function of surface treatment. The crystal structure of the nucleated Ca-P crystals had a diffraction pattern similar to that of hydroxyapatite, the mineralized component of bone. The study shows that the scaffold surface chemistry can be manipulated, providing numerous routes to engineer cellulosic substrates for the requirements of scaffolding.

biomimetic apatite

cellulose acetate

phosphate

calcium

biomedical applications

regenerated cellulose

carboxymethyl cellulose

bacterial cellulose

cells

tissue

scaffold

hydroxyapatite

electrospinning

fiber diameter

tissue engineering

cellulose

affinity membrane

acetate

Författare

K. Rodriguez

Virginia Polytechnic Institute and State University

S. Renneckar

Virginia Polytechnic Institute and State University

Paul Gatenholm

Chalmers, Kemi- och bioteknik, Polymerteknologi

ACS Applied Materials & Interfaces

1944-8244 (ISSN) 1944-8252 (eISSN)

Vol. 3 681-689

Ämneskategorier

Kemiteknik

DOI

10.1021/am100972r