The Antennapedia peptide penetratin translocates across lipid bilayers - the first direct observation
Artikel i vetenskaplig tidskrift, 2000

The potential use of polypeptides and oligonucleotides for therapeutical purposes has been questioned because of their inherently poor cellular uptake. However, the 16-mer oligopeptide penetratin, derived from the homeodomain of Antennapedia, has been reported to enter cells readily via a non-endocytotic and receptor- and transporter-independent pathway, even when conjugated to large hydrophilic molecules. We here present the first study where penetratin is shown to traverse a pure lipid bilayer. The results support the idea that the uptake mechanism involves only the interaction of the peptide with the membrane lipids. Furthermore, we conclude that the translocation does not involve pore formation.

antennapedia homeodomain



membrane translocation

delivery vector


Per Thoren

Institutionen för fysikalisk kemi

Daniel Persson

Institutionen för fysikalisk kemi

M. Karlsson

Bengt Nordén

Institutionen för fysikalisk kemi

FEBS Letters

0014-5793 (ISSN) 18733468 (eISSN)

Vol. 482 3 265-268


Biokemi och molekylärbiologi



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