Flow-alignment of bicellar lipid mixtures: orientations of probe molecules and membrane-associated biomacromolecules in lipid membranes studied with polarized light
Artikel i vetenskaplig tidskrift, 2011

Bicelles are excellent membrane-mimicking hosts for a dynamic and structural study of solutes with NMR, but the magnetic fields required for their alignment are hard to apply to optical conditions. Here we demonstrate that bicellar mixtures can be aligned by shear forces in a Couette flow cell, to provide orientation of membrane-bound retinoic acid, pyrene and cytochrome c (cyt c) protein, conveniently studied with linear dichroism spectroscopy.

nmr

proteins

model

linear dichroism spectroscopy

binding

micelles

Författare

Maxim Kogan

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

Tamas Beke-Somfai

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

Bengt Nordén

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

Chemical Communications

1359-7345 (ISSN)

Vol. 47 7356-7358

Styrkeområden

Energi

Livsvetenskaper och teknik

Materialvetenskap

Ämneskategorier

Kemi

DOI

10.1039/c1cc12313k