SEQUENCE DEPENDENCE OF 4',6-DIAMIDINO-2-PHENYLINDOLE (DAPI) DNA INTERACTIONS
Artikel i vetenskaplig tidskrift, 1993

We have characterized the interaction of 4',6-diamidino-2-phenylindole (DAPI) with a series of DNA oligonucleotides containing a varying number of contiguous alternating AT base pairs in the center of a GC-stretch. The binding affinity of DAPI to the oligonucleotides increases with the number of contiguous AT base pairs. When entropy effects are taken into account, the microscopic affinity reaches a maximum value when the oligonucleotides contain three AT base pairs. Three is also the minimum number of base pairs that provides a binding site with all the characteristics of binding observed with DNA and [poly(dA-dT)]2 at low binding ratios. With none or only a single AT base pair in the center of a GC dodecamer, the spectral properties of bound DAPI are very similar to those of DAPI bound to [poly(dG-dC)]2. When DAPI is complexed with an oligonucleotide containing two AT base pairs, the absorption and fluorescence properties of DAPI closely resemble those of DAPI complexed with [poly(dG-dC)]2, while the circular dichroism spectrum resembles that of DAPI bound to [poly(dA-dT)]2.

4'-6-diamidino-2-phenylindole

hoechst-33258

fluorescence

spectroscopy

dichroism

complexes

binding sites

Författare

Karl Jansen

Bengt Nordén

Institutionen för fysikalisk kemi

Mikael Kubista

Institutionen för biokemi och biofysik

Journal of the American Chemical Society

0002-7863 (ISSN) 1520-5126 (eISSN)

Vol. 115 23 10527-10530

Ämneskategorier

Kemi

DOI

10.1021/ja00076a009