ROLE OF TYROSINE RESIDUE-264 OF RECA FOR THE BINDING OF COFACTOR AND DNA
Artikel i vetenskaplig tidskrift, 1993

The tyrosine fluorescence of the RecA protein is quenched by about 15% upon binding of the cofactor analog adenosine 5'-O-(3-thiotriphosphate) (ATPgammaS). This quenching is not observed with a modified RecA in which the tyrosine residue at position 264 (Tyr-264) is replaced for alanine by site-directed mutagenesis, a modification which also results in a decrease of binding affinity of cofactor. This indicates that Tyr-264 is responsible for the fluorescence change and that the residue is close to or within the cofactor binding site. Upon DNA binding, a change of tyrosine fluorescence is observed both with the modified protein and with wild type RecA, indicating that DNA binding affects the environment of other tyrosine residues than Tyr-264. However, the change is significantly smaller in the modified protein, suggesting that both Tyr-264 as well as other residue(s) may be affected by the DNA binding. Changed fluorescence properties of the remaining tyrosine residues as a result of a slightly different DNA binding mode of the modified protein are also possible. Tyr-264 may be an important residue for the allosteric effect induced by the cofactor for the binding of DNA to RecA. In the recent crystal structure of RecA-ADP published by Story and Steitz (Story, R. M., and Steitz, T. A. (1992) Nature 355, 374-376), ADP is stacked with Tyr-103 and does not interact with Tyr-264. The fact that we observe no interaction of ATPgammaS with Tyr-103 (as evidenced from absence of fluorescence change) but instead with Tyr-264 may suggest an important conformational difference between the RecA complexes with, respectively, ADP and ATP.

atp hydrolysis

protein

electron-microscopy

nucleotide cofactor

general

single-stranded-dna

escherichia-coli

recombination

lambda-repressor

linear dichroism

complexes

Författare

Svante Eriksson

Institutionen för fysikalisk kemi

Bengt Nordén

Institutionen för fysikalisk kemi

Katsumi Morimatsu

T. Horii

Masayuki Takahashi

Journal of Biological Chemistry

0021-9258 (ISSN) 1083-351X (eISSN)

Vol. 268 1811-1816

Ämneskategorier

Biokemi och molekylärbiologi