METHYL GREEN - A DNA MAJOR-GROOVE BINDING-DRUG
Artikel i vetenskaplig tidskrift, 1993

Interaction and binding geometries of complexes of Methyl green with poly(dA-dT)2, poly(dA) . poly(dT), and triplex poly(dA) . 2poly(dT) complexes have been studied by linear dichroism. For both of the complexes with double helical DNAs, the z symmetry axis of Methyl green is found to be approximately parallel to the DNA bases while the x symmetry axis lies at 40-44-degrees relative to the local DNA helix axis, in agreement with a groove binding mode. However, in contrast to minor-groove binders (such as DAPI and Hoechst 33258) Methyl green is found to be excluded from binding to the triple helical poly(dA) . 2poly(dT) in which the major groove is filled by the third strand. While most so far studied groove-binding dyes bind in the minor groove of DNA, Methyl green thus appears to be an exception.

triplex

dna

poly(da).2poly(dt)

linear dichroism

poly(dg-dc)

methyl green

poly(da).poly(dt)

major groove binding

Författare

Seog K. Kim

Institutionen för fysikalisk kemi

Bengt Nordén

Institutionen för fysikalisk kemi

FEBS Letters

0014-5793 (ISSN)

Vol. 315 1 61-64

Ämneskategorier

Biokemi och molekylärbiologi

DOI

10.1016/0014-5793(93)81133-k