STRUCTURE OF UVRABC EXCINUCLEASE UV-DAMAGED DNA COMPLEXES STUDIED BY FLOW LINEAR DICHROISM - DNA CURVED BY UVRB AND UVRC
Artikel i vetenskaplig tidskrift, 1992

The interaction between UvrABC excinuclease from Escherichia coli and ultraviolet light- (UV) damaged DNA was studied by flow linear dichroism. The dichroism signal from DNA was drastically decreased in intensity upon incubation with UvrA and UvrB or whole enzyme in the presence of effector ATP. The change was specific for UV-damaged DNA, and a concluded suppressed DNA orientation suggests the wrapping of DNA around the protein. The incubation with the UvrC subunit alone also somewhat reduces the signal, however, in this case the change was smaller and not specific for UV-damaged DNA. The structural modification of DNA, promoted by the (UvrA2-UvrB) complex, probably facilitates or stabilizes the interaction of the UvrC subunit with DNA for the excision.

dna repair

uvrabc excinuclease

protein

uv damaged

binding

escherichia-coli

protein dna complex

dna

linear dichroism

repair

Författare

Masayuki Takahashi

E. Bertrandburggraf

R. P. P. Fuchs

Bengt Nordén

Institutionen för fysikalisk kemi

FEBS Letters

0014-5793 (ISSN)

Vol. 314 1 10-12

Ämneskategorier

Biokemi och molekylärbiologi

Cell- och molekylärbiologi

DOI

10.1016/0014-5793(92)81448-u