Studying the ability of Fusarium oxysporum and recombinant Saccharomyces cerevisiae to efficiently cooperate in decomposition and ethanolic fermentation of wheat straw
Artikel i vetenskaplig tidskrift, 2011

Fusarium oxysporum F3 alone or in mixed culture with Saccharomyces cerevisiae F12 were used to ferment carbohydrates of wet exploded pre-treated wheat straw (PWS) directly to ethanol. Both microorganisms were first grown aerobically to produce cell mass and thereafter fermented PWS to ethanol under anaerobic conditions. During fermentation, soluble and insoluble carbohydrates were hydrolysed by the lignocellulolytic system of F. oxysporum. Mixed substrate fermentation using PWS and corn cobs (CC) in the ratio 1:2 was used to obtain an enzyme mixture with high cellulolytic and hemicellulolytic activities. Under these conditions, activities as high as 34300, 9100, 326, 24, 169, 27 and 254 U dm(-3) of xylanase, endoglucanase, beta-glucosidase, arabinofuranosidase, avicelase, feruloyl esterase and acetyl esterase, respectively, were obtained. The replacement of the enzyme production phase of F. oxysporum by the addition of commercially available enzymes Celluclast (R) 1.5 L FG and Novozym (R) 188 in 3:1 ratio for the treatment of PWS, resulted in a 3-fold increase in the volumetric ethanol productivity without increasing the ethanol production significantly. By direct bioconversion of 110 kg m(-3) dry matter of PWS, ethanol concentration (4.9 kg m(-3)) and yield (40 g kg(-1) of PWS) were similarly obtained by F. oxysporum and the mixed culture, while productivity rates as high as 34 g m(-3) h(-1) and 108 g m(-3) h(-1) were obtained by F. oxysporum and the mixed culture, respectively.

hemicellulose

Ethanol

cellulose

Wheat straw

Consolidated

esterase

purification

bioprocessing

Saccharomyces cerevisiae

wet-oxidation

performance

direct conversion

pretreatment

Fusarium oxysporum

solid-state fermentation

hydrolysis

Författare

G. Panagiotou

Danmarks Tekniske Universitet (DTU)

E. Topakas

National Technical University of Athens (NTUA)

M. Moukouli

National Technical University of Athens (NTUA)

P. Christakopoulos

National Technical University of Athens (NTUA)

Lisbeth Olsson

Chalmers, Kemi- och bioteknik, Industriell Bioteknik

Biomass and Bioenergy

0961-9534 (ISSN)

Vol. 35 8 3727-3732

Ämneskategorier

Industriell bioteknik

DOI

10.1016/j.biombioe.2011.05.005