Bacterial cellulose modified with xyloglucan bearing the adhesion peptide RGD promotes endothelial cell adhesion and metabolism--a promising modification for vascular grafts.
Artikel i vetenskaplig tidskrift, 2011

Today, biomaterials such as polytetrafluorethylene (ePTFE) are used clinically as prosthetic grafts for vascular surgery of large vessels (>5 mm). In small diameter vessels, however, their performance is poor due to early thrombosis. Bacterial-derived cellulose (BC) is a new promising material as a replacement for blood vessels. This material is highly biocompatible in vivo but shows poor cell adhesion. In the native blood vessel, the endothelium creates a smooth non-thrombogenic surface. In order to sustain cell adhesion, BC has to be modified. With a novel xyloglucan (XG) glycoconjugate method, it is possible to introduce the cell adhesion peptide RGD (Arg-Gly-Asp) onto bacterial cellulose. The advantage of the XG-technique is that it is an easy one-step procedure carried out in water and it does not weaken or alter the fiber structure of the hydrogel. In this study, BC was modified with XG and XGRGD to asses primary human vascular endothelial cell adhesion, proliferation, and metabolism as compared with unmodified BC. This XG-RGD-modification significantly increased cell adhesion and the metabolism of seeded primary endothelial cells as compared with unmodified BC whereas the proliferation rate was affected only to some extent. The introduction of an RGD-peptide to the BC surface further resulted in enhanced cell spreading with more pronounced stress fiber formation and mature phenotype. This makes BC together with the XG-method a promising material for synthetic grafts in vascular surgery and cardiovascular research.

pharmacology

pharmacology

Vascular Grafting

drug effects

Cell Shape

drug effects

metabolism

Cell Movement

Glucans

drug effects

Humans

Fluorescence

Cell Proliferation

cytology

Xylans

Cell Adhesion

Serum

Cellulose

chemistry

pharmacology

Staining and Labeling

methods

Oligopeptides

Endothelial Cells

metabolism

Microscopy

Stress Fibers

drug effects

Phase-Contrast

drug effects

Cattle

Gluconacetobacter xylinus

pharmacology

drug effects

Animals

Författare

Helen Fink

Göteborgs universitet

Lage Ahrenstedt

Kungliga Tekniska Högskolan (KTH)

Aase Katarina Bodin

Chalmers, Kemi- och bioteknik, Polymerteknologi

Harry Brumer

Kungliga Tekniska Högskolan (KTH)

Paul Gatenholm

Chalmers, Kemi- och bioteknik, Polymerteknologi

Wallenberg Wood Science Center (WWSC)

Alexandra Krettek

Göteborgs universitet

Bo Risberg

Göteborgs universitet

Journal of tissue engineering and regenerative medicine

1932-7005 (eISSN)

Vol. 5 6 454-63

Ämneskategorier

MEDICIN OCH HÄLSOVETENSKAP

DOI

10.1002/term.334

PubMed

21604383