Bacterial cellulose modified with xyloglucan bearing the adhesion peptide RGD promotes endothelial cell adhesion and metabolism--a promising modification for vascular grafts.
Artikel i vetenskaplig tidskrift, 2011
Today, biomaterials such as polytetrafluorethylene (ePTFE) are used clinically as prosthetic grafts for vascular surgery of large vessels (>5 mm). In small diameter vessels, however, their performance is poor due to early thrombosis. Bacterial-derived cellulose (BC) is a new promising material as a replacement for blood vessels. This material is highly biocompatible in vivo but shows poor cell adhesion. In the native blood vessel, the endothelium creates a smooth non-thrombogenic surface. In order to sustain cell adhesion, BC has to be modified. With a novel xyloglucan (XG) glycoconjugate method, it is possible to introduce the cell adhesion peptide RGD (Arg-Gly-Asp) onto bacterial cellulose. The advantage of the XG-technique is that it is an easy one-step procedure carried out in water and it does not weaken or alter the fiber structure of the hydrogel. In this study, BC was modified with XG and XGRGD to asses primary human vascular endothelial cell adhesion, proliferation, and metabolism as compared with unmodified BC. This XG-RGD-modification significantly increased cell adhesion and the metabolism of seeded primary endothelial cells as compared with unmodified BC whereas the proliferation rate was affected only to some extent. The introduction of an RGD-peptide to the BC surface further resulted in enhanced cell spreading with more pronounced stress fiber formation and mature phenotype. This makes BC together with the XG-method a promising material for synthetic grafts in vascular surgery and cardiovascular research.
pharmacology
pharmacology
Vascular Grafting
drug effects
Cell Shape
drug effects
metabolism
Cell Movement
Glucans
drug effects
Humans
Fluorescence
Cell Proliferation
cytology
Xylans
Cell Adhesion
Serum
Cellulose
chemistry
pharmacology
Staining and Labeling
methods
Oligopeptides
Endothelial Cells
metabolism
Microscopy
Stress Fibers
drug effects
Phase-Contrast
drug effects
Cattle
Gluconacetobacter xylinus
pharmacology
drug effects
Animals
Författare
Helen Fink
Göteborgs universitet
Lage Ahrenstedt
Kungliga Tekniska Högskolan (KTH)
Aase Katarina Bodin
Chalmers, Kemi- och bioteknik, Polymerteknologi
Harry Brumer
Kungliga Tekniska Högskolan (KTH)
Paul Gatenholm
Chalmers, Kemi- och bioteknik, Polymerteknologi
Wallenberg Wood Science Center (WWSC)
Alexandra Krettek
Göteborgs universitet
Bo Risberg
Göteborgs universitet
Journal of tissue engineering and regenerative medicine
1932-7005 (eISSN)
Vol. 5 6 454-63Ämneskategorier
MEDICIN OCH HÄLSOVETENSKAP
DOI
10.1002/term.334
PubMed
21604383