Design and characterization of a novel amphiphilic chitosan nanocapsule-based thermo-gelling biogel with sustained in vivo release of the hydrophilic anti-epilepsy drug ethosuximide
Artikel i vetenskaplig tidskrift, 2012

Thermo-gelling injectable nanogels, with no burst release of loaded drug, were prepared by a simple route by combining self assembled nanocapsules of amphiphilically modified chitosan with glycerophosphate di-sodium salt and glycerol. The potential as a depot drug delivery system was demonstrated in vivo through the therapeutic effect of ethosuximide (ESM) loaded nanogels, suppressing spike wave discharges (SWDs) in Long Evan rat model. Simultaneously clearance of gels from the site of administration was monitored non-invasively using MRI. The gel structure was characterized using TEM and SEM, confirming the gels to be an assembly of nanocapsules and using two-photon microscopy to visualize the network structure. In vitro drug release studies using ESM revealed that the nanogels exhibited extended, mostly Fickian release. Finally, all investigated formulations displayed excellent cytotoxicity data determined by MTT assay using human retinal pigmented epithelium cells. All presented properties are highly desirable for injectable depot gels for drug delivery.

In vivo

Thermo responsive

Drug delivery

Nano capsules

Amphiphilic chitosan

Författare

Meng-Hsuan Hsiao

National Chiao Tung University Taiwan

Mikael Larsson

SuMo Biomaterials

Chalmers, Kemi- och bioteknik, Farmaceutisk teknologi

Anette Larsson

Chalmers, Kemi- och bioteknik, Farmaceutisk teknologi

SuMo Biomaterials

Hanne Evenbratt

Chalmers, Kemi- och bioteknik, Farmaceutisk teknologi

Ying-Yu Chen

University of Southern California

You-Yin Chen

National Yang-Ming University Taiwan

D. M. Liu

National Chiao Tung University Taiwan

Journal of Controlled Release

0168-3659 (ISSN)

Vol. 161 3 942-948

Styrkeområden

Nanovetenskap och nanoteknik

Materialvetenskap

Ämneskategorier

Farmaceutisk vetenskap

Annan medicinsk grundvetenskap

Kemi

DOI

10.1016/j.jconrel.2012.05.038

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Skapat

2017-10-07