Fast and accurate preparation fatty acid methyl esters by microwave-assisted derivatization in the yeast Saccharomyces cerevisiae
Artikel i vetenskaplig tidskrift, 2012

We present a fast and accurate method for preparation of fatty acid methyl esters (FAMEs) using microwave-assisted derivatization of fatty acids present in yeast samples. The esterification of free/bound fatty acids to FAMEs was completed within 5 min, which is 24 times faster than with conventional heating methods. The developed method was validated in two ways: (1) through comparison with a conventional method (hot plate) and (2) through validation with the standard reference material (SRM) 3275-2 omega-3 and omega-6 fatty acids in fish oil (from the Nation Institute of Standards and Technology, USA). There were no significant differences (P > 0.05) in yields of FAMEs with both validations. By performing a simple modification of closed-vessel microwave heating, it was possible to carry out the esterification in Pyrex glass tubes kept inside the closed vessel. Hereby, we are able to increase the number of sample preparations to several hundred samples per day as the time for preparation of reused vessels was eliminated. Pretreated cell disruption steps are not required, since the direct FAME preparation provides equally quantitative results. The new microwave-assisted derivatization method facilitates the preparation of FAMEs directly from yeast cells, but the method is likely to also be applicable for other biological samples.

Saccharomyces

cell

gene

Microwave-assisted derivatization

extraction

chromatography/mass spectrometry analysis

direct transesterification

Fatty acid analysis

lipids

Fatty acid methyl esters

samples

Författare

Sakda Khoomrung

Chalmers, Kemi- och bioteknik, Livsvetenskaper, Systembiologi

Pramote Chumnanpuen

Chalmers, Kemi- och bioteknik, Livsvetenskaper, Systembiologi

Suwanee Jansa-Ard

Chalmers, Kemi- och bioteknik, Livsvetenskaper, Systembiologi

Intawat Nookaew

Chalmers, Kemi- och bioteknik, Livsvetenskaper, Systembiologi

Jens B Nielsen

Chalmers, Kemi- och bioteknik, Livsvetenskaper, Systembiologi

Applied Microbiology and Biotechnology

0175-7598 (ISSN) 1432-0614 (eISSN)

Vol. 94 1637-1646

Ämneskategorier

Industriell bioteknik

Mikrobiologi

Styrkeområden

Livsvetenskaper och teknik

DOI

10.1007/s00253-012-4125-x