In vitro characterization and osteoblast responses to nanostructured photocatalytic TiO2 coated surfaces.
Artikel i vetenskaplig tidskrift, 2012

The aims of the study were to characterize a nanostructured photoactive titanium dioxide (TiO(2)) coating and to compare the cellular response of human osteoblasts before and after ultraviolet (UV) irradiation of the coating. A specific nanostructured TiO(2) powder (Degussa P-25), which consists of approximately 80% anatase and 20% rutile, was spin-coated onto commercially pure titanium discs, and was heat-treated thereafter. After topographical, chemical and photocatalytic property characterizations, human osteoblasts were cultured on the coated discs before and after UV irradiation. Cell morphology was evaluated by scanning electron microscopy (SEM), and cell viability was analysed by 3-(4,5-dimethylthiazol)-2,5-diphenyltetrazolium bromide (MTT) assay. From the contact angle analysis, the wettability significantly improved after UV irradiation. The cultured cells were flattened with numerous elongated lammellipodia; however, no morphological differences were indicated between -UV and +UV surfaces. The MTT assay analysis showed that -UV surface presented significantly higher viability compared to the +UV surface except for one cell population group at 3h where there were no differences. The nanostructured photoactive TiO(2) surface improved its hydrophilicity by UV irradiation, however no enhancing effect in cell response was confirmed at the time tested compared to the non-irradiated surface.

Photocatalytic properties

Anatase

Hydrophilicity

Osteoblasts

Nanostructures

Författare

Mariko Hayashi

Malmö universitet

Ryo Jimbo

Malmö universitet

Liselott Lindh

Malmö universitet

Javier Sotres

Malmö universitet

Takashi Sawase

Nagasaki University

K. Mustafa

Universitetet i Bergen

Martin Andersson

Chalmers, Kemi- och bioteknik, Teknisk ytkemi

Ann Wennerberg

Malmö universitet

Acta Biomaterialia

1742-7061 (ISSN)

Vol. 8 2411-6

Ämneskategorier

Biomaterialvetenskap

DOI

10.1016/j.actbio.2012.03.010

PubMed

22409872