Hemocompatibility of siRNA loaded dextran nanogels
Artikel i vetenskaplig tidskrift, 2011

Although the behavior of nanoscopic delivery systems in blood is an important parameter when contemplating their intravenous injection, this aspect is often poorly investigated when advancing from in vitro to in vivo experiments. In this paper, the behavior of siRNA loaded dextran nanogels in human plasma and blood is examined using fluorescence fluctuation spectroscopy, platelet aggregometry, flow cytometry and single particle tracking. Our results show that, in contrast to their negatively charged counterparts, positively charged siRNA loaded dextran nanogels cause platelet aggregation and show increased binding to human blood cells. Although PEGylating the nanogels did not have a significant effect on their interaction with blood cells, single particle tracking revealed that it is necessary to prevent their aggregation in human plasma. We therefore conclude that PEGylated negatively charged dextran nanogels are the most suited for further in vivo studies as they do not aggregate in human plasma and exhibit minimal interactions with blood cells.

nanoparticle

drug delivery

haemocompatibility

blood compatibility

blood

gene therapy

Författare

B Naeye

Universiteit Gent

H Deschout

Universiteit Gent

Magnus Röding

Chalmers, Matematiska vetenskaper, Matematisk statistik

SuMo Biomaterials

Göteborgs universitet

Mats Rudemo

Göteborgs universitet

Chalmers, Matematiska vetenskaper, Matematisk statistik

J Delanghe

Center for Medical Genetics

K Devreese

Center for Medical Genetics

J Demeester

Universiteit Gent

K Braeckmans

Universiteit Gent

SC De Smedt

Universiteit Gent

K Raemdonck

Universiteit Gent

Biomaterials

0142-9612 (ISSN)

Vol. 32 34 9120-9127

Ämneskategorier

Biomaterialvetenskap

DOI

10.1016/j.biomaterials.2011.08.015

PubMed

21890194

Mer information

Senast uppdaterat

2020-02-19