Evolutionary engineering of Saccharomyces cerevisiae for efficient aerobic xylose consumption
Artikel i vetenskaplig tidskrift, 2012

Industrial biotechnology aims to develop robust microbial cell factories, such as Saccharomyces cerevisiae, to produce an array of added value chemicals presently dominated by petrochemical processes. Xylose is the second most abundant monosaccharide after glucose and the most prevalent pentose sugar found in lignocelluloses. Significant research efforts have focused on the metabolic engineering of S similar to cerevisiae for fast and efficient xylose utilization. This study aims to metabolically engineer S similar to cerevisiae, such that it can consume xylose as the exclusive substrate while maximizing carbon flux to biomass production. Such a platform may then be enhanced with complementary metabolic engineering strategies that couple biomass production with high value-added chemical. Saccharomyces cerevisiae, expressing xylose reductase, xylitol dehydrogenase and xylulose kinase, from the native xylose-metabolizing yeast Pichia stipitis, was constructed, followed by a directed evolution strategy to improve xylose utilization rates. The resulting S similar to cerevisiae strain was capable of rapid growth and fast xylose consumption producing only biomass and negligible amount of byproducts. Transcriptional profiling of this strain was employed to further elucidate the observed physiology confirms a strongly up-regulated glyoxylate pathway enabling respiratory metabolism. The resulting strain is a desirable platform for the industrial production of biomass-related products using xylose as a sole carbon source.

xylitol dehydrogenase

pichia-stipitis

chemostat cultures

ethanol-production

cytosolic nadh

directed evolution

metabolic engineering

reductase-activity

gene-expression

Saccharomyces

xylose

transcriptional regulation

pentose-phosphate pathway

genome database

Författare

Gionata Scalcinati

Chalmers, Kemi- och bioteknik, Livsvetenskaper, Systembiologi

José Manuel Otero

Chalmers, Kemi- och bioteknik, Livsvetenskaper, Systembiologi

J. R. H. Van Vleet

University of Wisconsin Madison

T. W. Jeffries

University of Wisconsin Madison

USDA Forest Products Laboratory

Lisbeth Olsson

Chalmers, Kemi- och bioteknik, Industriell Bioteknik

Jens B Nielsen

Chalmers, Kemi- och bioteknik, Livsvetenskaper, Systembiologi

FEMS Yeast Research

1567-1356 (ISSN) 1567-1364 (eISSN)

Vol. 12 582-597

Drivkrafter

Hållbar utveckling

Styrkeområden

Energi

Livsvetenskaper och teknik

Ämneskategorier

Kemiteknik

DOI

10.1111/j.1567-1364.2012.00808.x