Advances in Quantitative FRET-Based Methods for Studying Nucleic Acids
Artikel i vetenskaplig tidskrift, 2012

Förster resonance energy transfer (FRET) is a powerful tool for monitoring molecular distances and interactions at the nanoscale level. The strong dependence of transfer efficiency on probe separation makes FRET perfectly suited for on/off experiments. To use FRET to obtain quantitative distances and three-dimensional structures, however, is more challenging. This review summarises recent studies and technological advances that have improved FRET as a quantitative molecular ruler in nucleic acid systems, both at the ensemble and at the single-molecule levels.

photon distribution analysis

photoinduced electron-transfer

multiparameter fluorescence detection

alternating-laser

rna-polymerase-ii

resonance energy-transfer

FRET

nucleic acids

DNA structures

excitation

double-stranded dna

fluorescence spectroscopy

analysis

probability-distribution

fluorescent probes

single-molecule fret

nano-positioning system

Författare

S. Preus

Kobenhavns Universitet

Marcus Wilhelmsson

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

ChemBioChem

1439-4227 (ISSN) 1439-7633 (eISSN)

Vol. 13 1990-2001

Styrkeområden

Nanovetenskap och nanoteknik

Livsvetenskaper och teknik

Ämneskategorier

Fysikalisk kemi

Biokemi och molekylärbiologi

Biofysik

Kemi

DOI

10.1002/cbic.201200400