A New Modular Approach to Nanoassembly: Stable and Addressable DNA Nanoconstructs via Orthogonal Click Chemistries
Artikel i vetenskaplig tidskrift, 2012

Thermodynamic instability is a problem when assembling and purifying complex DNA nanostructures formed by hybridization alone. To address this issue, we have used photochemical fixation and orthogonal copper-free, ring-strain-promoted, click chemistry for the synthesis of dimeric, trimeric, and oligomeric modular DNA scaffolds from cyclic, double-stranded, 80-mer DNA nanoconstructs. This particular combination of orthogonal click reactions was more effective for nanoassembly than others explored. The complex nanostructures are stable to heat and denaturation agents and can therefore be purified and characterized. They are addressable in a sequence-specific manner by triplex formation, and they can be reversibly and selectively deconstructed. Nanostructures utilizing this orthogonal, chemical fixation methodology can be used as building blocks for nanomachines and functional DNA nanoarchitectures.

DNA nanotechnology

nanostructures

photochemical cross-linking

copper-free click

stability

template

dip-pen nanolithography

orthogonal chemical fixation

gold nanoparticles

photo-cross-linking

design

oligomers

Författare

S. R. Gerrard

University of Southampton

C. Hardiman

University of Southampton

M. Shelbourne

University of Southampton

I. S. Nandhakumar

University of Southampton

Bengt Nordén

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

T. Brown

University of Southampton

ACS Nano

1936-0851 (ISSN) 1936-086X (eISSN)

Vol. 6 9221-9228

Styrkeområden

Nanovetenskap och nanoteknik

Energi

Livsvetenskaper och teknik

Ämneskategorier

Kemi

DOI

10.1021/nn3035759

PubMed

22989197