Mechanically robust polysiloxane – ACA capsules for prolonged ethanol production
Artikel i vetenskaplig tidskrift, 2012

Background Fermentation using encapsulated yeast leads to more robust ethanol production from lignocellulose hydrolyzates. Encapsulated yeast is much more tolerant to inhibitors present in hydrolyzates, and fermentation is faster due to increased total cell density. For industrial applications, capsules must be made robust enough to endure long periods and numerous cultivations without breaking. Results Liquid core alginatechitosanalginate (ACA) capsules containing Saccharomyces cerevisiae were produced by the liquid-droplet-forming method and treated with hydrolyzed 3-aminopropyltrietoxysilane (hAPTES) forming very glossy capsules. Capsules produced with 3.0% hAPTES showed the best mechanical robustness but no ethanol could be produced in dilute-acid spruce hydrolyzate using these capsules. Untreated ACA capsules gave the highest ethanol production but demonstrated poor mechanical robustness. 25% of the ACA capsules ruptured within 6 h in the shear test. Capsules treated with 1.5% hAPTES were significantly stronger, since only 02% of these capsules broke. Moreover, the ethanol production in the fifth consecutive cultivation in lignocellulose hydrolyzate was nearly as high as for untreated ACA capsules. Conclusion The mechanical robustness of ACA capsules can be easily improved by treating the capsules with hAPTES. ACA capsules treated with 1.5% hAPTES showed excellent mechanical robustness and a similar ethanol production profile to untreated ACA capsules.

robust capsules

encapsulation

yeast

3-aminopropyltrietoxysilane

alginate

chitosan

bioethanol production

Författare

Päivi Ylitervo

Chalmers, Kemi- och bioteknik, Industriell Bioteknik

Carl Johan Franzén

Chalmers, Kemi- och bioteknik, Industriell Bioteknik

Mohammad Taherzadeh Esfahani

Högskolan i Borås

Journal of Chemical Technology and Biotechnology

0268-2575 (ISSN)

Vol. 88 1080-1088

Drivkrafter

Hållbar utveckling

Ämneskategorier

Industriell bioteknik

DOI

10.1002/jctb.3944