MicroRNA regulate immunological pathways in T-cells in immune thrombocytopenia (ITP)
Artikel i vetenskaplig tidskrift, 2013

MicroRNA are small non-coding RNA molecules that regulate gene expression. To investigate the role of microRNA in ITP, we performed genome-wide expression analyses of mRNA and microRNA in T-cells from ITP patients and controls. We identified 1,915 regulated genes and 22 regulated microRNA that differed between ITP patients and controls. Seventeen of the 22 regulated microRNA were linked to changes in target gene expression; 57 of these target genes were associated with the immune system, e.g. T-cell activation and regulation of immunoglobulin production. CXCL13 and IL-21 were two microRNA target genes significantly increased in ITP. We could demonstrate increased plasma levels of CXCL13 and others have reported increased plasma levels of IL-21 in ITP. Thus, regulated microRNA were significantly associated with both gene and protein expression of molecules in immunological pathways. We suggest that microRNA may be important regulatory molecules involved in the loss of tolerance in ITP.

Gene Expression Profiling

Cluster Analysis

Microarray Analysis

Humans

MicroRNAs

Case-Control Studies

Författare

Margareta Jernås

Göteborgs universitet

Intawat Nookaew

Chalmers, Kemi- och bioteknik, Livsvetenskaper, Systembiologi

Hans Wadenvik

Göteborgs universitet

Bob Olsson

Göteborgs universitet

Blood

0006-4971 (ISSN) 1528-0020 (eISSN)

Vol. 121 11 2095-2098

Ämneskategorier

Hematologi

DOI

10.1182/blood-2012-12-471250

PubMed

23303824