Clinical dyslipidaemia is associated with changes in the lipid composition and inflammatory properties of apolipoprotein-B-containing lipoproteins from women with type 2 diabetes.
Artikel i vetenskaplig tidskrift, 2012

Aims/hypothesis The aim of this study was to use lipidomics to determine if the lipid composition of apolipoprotein-B-containing lipoproteins is modified by dyslipidaemia in type 2 diabetes and if any of the identified changes potentially have biological relevance in the pathophysiology of type 2 diabetes. Methods VLDL and LDL from normolipidaemic and dyslipidaemic type 2 diabetic women and controls were isolated and quantified with HPLC and mass spectrometry. A detailed molecular characterisation of VLDL triacylglycerols (TAG) was also performed using the novel ozone-induced dissociation method, which allowed us to distinguish vaccenic acid (C18:1 n-7) from oleic acid (C18:1 n-9) in specific TAG species. Results Lipid class composition was very similar in VLDL and LDL from normolipidaemic type 2 diabetic and control participants. By contrast, dyslipidaemia was associated with significant changes in both lipid classes (e.g. increased diacylglycerols) and lipid species (e.g. increased C16:1 and C20:3 in phosphatidylcholine and cholesteryl ester and increased C16:0 [palmitic acid] and vaccenic acid in TAG). Levels of palmitic acid in VLDL and LDL TAG correlated with insulin resistance, and VLDL TAG enriched in palmitic acid promoted increased secretion of proinflammatory mediators from human smooth muscle cells. Conclusions We showed that dyslipidaemia is associated with major changes in both lipid class and lipid species composition in VLDL and LDL from women with type 2 diabetes. In addition, we identified specific molecular lipid species that both correlate with clinical variables and are proinflammatory. Our study thus shows the potential of advanced lipidomic methods to further understand the pathophysiology of type 2 diabetes.

Type 2

physiopathology

Lipoproteins

Humans

Apolipoproteins B

Lipoproteins

metabolism

physiopathology

Middle Aged

metabolism

LDL

complications

Diabetes Mellitus

metabolism

VLDL

complications

physiopathology

Female

Dyslipidemias

Inflammation

metabolism

metabolism

metabolism

Författare

Marcus Ståhlman

Göteborgs universitet

H T Pham

Martin Adiels

Göteborgs universitet

Chalmers, Matematiska vetenskaper

T W Mitchell

S J Blanksby

Björn Fagerberg

Göteborgs universitet

Kim Ekroos

Jan Borén

Göteborgs universitet

Diabetologia

0012-186X (ISSN) 1432-0428 (eISSN)

Vol. 55 4 1156-66

Ämneskategorier

Klinisk medicin

DOI

10.1007/s00125-011-2444-6

PubMed

22252473

Mer information

Skapat

2017-10-06