Incorporating molecular data in fungal systematics: a guide for aspiring researchers
Artikel i vetenskaplig tidskrift, 2013

The last twenty years have witnessed molecular data emerge as a primary research instrument in most branches of mycology. Fungal systematics, taxonomy, and ecology have all seen tremendous progress and have undergone rapid, far-reaching changes as disciplines in the wake of continual improvement in DNA sequencing technology. A taxonomic study that draws from molecular data involves a long series of steps, ranging from taxon sampling through the various laboratory procedures and data analysis to the publication process. All steps are important and influence the results and the way they are perceived by the scientific community. The present paper provides a reflective overview of all major steps in such a project with the purpose to assist research students about to begin their first study using DNA-based methods. We also take the opportunity to discuss the role of taxonomy in biology and the life sciences in general in the light of molecular data. While the best way to learn molecular methods is to work side by side with someone experienced, we hope that the present paper will serve to lower the learning threshold for the reader.

biodiversity – molecular marker – phylogeny – publication – species – Sanger sequencing – taxonomy

Författare

Kevin D. Hyde

Dhanushka Udayanga

Dimuthu S. Manamgoda

Leho Tedersoo

Ellen Larsson

Göteborgs universitet

Kessy Abarenkov

Yann Bertrand

Göteborgs universitet

Bengt Oxelman

Göteborgs universitet

Martin Hartmann

Håvard Kauserud

Martin Ryberg

Erik Kristiansson

Göteborgs universitet

Chalmers, Matematiska vetenskaper, matematisk statistik

R. Henrik Nilsson

Göteborgs universitet

Current Research in Environmental and Applied Mycology

2229-2225 (ISSN)

Vol. 3 1 artikel nr 1-

Ämneskategorier

Evolutionsbiologi

Botanik

Biologisk systematik

Markvetenskap

Ekologi

Mikrobiologi

Bioinformatik (beräkningsbiologi)

Mikrobiologi inom det medicinska området

Bioinformatik och systembiologi

DOI

10.5943/cream/3/1/1

Mer information

Skapat

2017-10-07