Bridging Ligand Length Controls AT Selectivity and Enantioselectivity of Binuclear Ruthenium Threading Intercalators
Artikel i vetenskaplig tidskrift, 2013

The slow dissociation of DNA threading intercalators makes them interesting as model compounds in the search for new DNA targeting drugs, as there appears to be a correlation between slow dissociation and biological activity. Thus, it would be of great value to understand the mechanisms controlling threading intercalation, and for this purpose we have investigated how the length of the bridging ligand of binuclear ruthenium threading intercalators affects their DNA binding properties. We have synthesised a new binuclear ruthenium threading intercalator with slower dissociation kinetics from ct-DNA than has ever been observed for any ruthenium complex with any type of DNA, a property that we attribute to the increased distance between the ruthenium centres of the new complex. By comparison with previously studied ruthenium complexes, we further conclude that elongation of the bridging ligand reduces the sensitivity of the threading interaction to DNA flexibility, resulting in a decreased AT selectivity for the new complex. We also find that the length of the bridging ligand affects the enantioselectivity with increasing preference for the enantiomer as the bridging ligand becomes longer.

kinetics

ruthenium

DNA

intercalation

sequence selectivity

Författare

Johan Johansson

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

Y. B. Wang

Xinjiang Medical University

Mattias P Eng

Göteborgs universitet

Nina Kann

Chalmers, Kemi- och bioteknik, Organisk kemi

Per Lincoln

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

Johanna Andersson

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

Chemistry - A European Journal

0947-6539 (ISSN) 1521-3765 (eISSN)

Vol. 19 6246-6256

Ämneskategorier

Fysikalisk kemi

DOI

10.1002/chem.201300483