Understanding the pH-dependent immobilization efficacy of feruloyl esterase-C on mesoporous silica and its structure activity changes
Artikel i vetenskaplig tidskrift, 2013

The purpose of the present investigation was to study the pH dependence of both the immobilization process and the enzyme activity of a feruloyl esterase (FoFaeC from Fusarium oxysporum) immobilized in mesoporous silica. This was done by interpreting experimental results with theoretical molecular modeling of the enzyme structure. Modeling of the 3D structure of the enzyme together with calculations of the electrostatic surface potential showed that changes in the electrostatic potential of the protein surface were correlated with the pH dependence of the immobilization process. High immobilization yields were associated with an increase in pH. The transesterification activity of both immobilized and free enzyme was studied at different values of pH and the optimal pH of the immobilized enzyme was found to be one unit lower than that for the free enzyme. The surface charge distribution around the binding pocket was identified as being a crucial factor for the accessibility of the active site of the immobilized enzyme, indicating that the orientation of the enzyme inside the pores is pH dependent. Interestingly, it was observed that the immobilization pH affects the specific activity, irrespective of the changes in reaction pH. This was identified as a pH memory effect for the immobilized enzyme. On the other hand, a change in product selectivity of the immobilized enzyme was also observed when the transesterification reaction was run in MOPS buffer instead of citrate phosphate buffer. Molecular docking studies revealed that the MOPS buffer molecule can bind to the enzyme binding pocket, and can therefore be assumed to modulate the product selectivity of the immobilized enzyme toward transesterification.


Mesoporous silica

Molecular simulations

Feruloyl esterases

Enzyme immobilization


Christian Thörn

Chalmers, Kemi- och bioteknik, Industriell Bioteknik

Gupta Udatha

Chalmers, Kemi- och bioteknik, Industriell Bioteknik

H. Zhou

Dalian University of Technology

P. Christakopoulos

Lulea tekniska Universitet

E. Topakas

National Technical University of Athens

Lisbeth Olsson

Chalmers, Kemi- och bioteknik, Industriell Bioteknik

Journal of Molecular Catalysis - B Enzymatic

1381-1177 (ISSN)

Vol. 93 65-72


Industriell bioteknik