Formation of supported lipid bilayers on silica: relation to lipid phase transition temperature and liposome size
Artikel i vetenskaplig tidskrift, 2014

DPPC liposomes ranging from 90 nm to 160 nm in diameter were prepared and used for studies of the formation of supported lipid membranes on silica (SiO2) at temperatures below and above the gel to liquid-crystalline phase transition temperature (T-m = 41 degrees C), and by applying temperature gradients through T-m. The main method was the quartz crystal microbalance with dissipation (QCM-D) technique. It was found that liposomes smaller than 100 nm spontaneously rupture on the silica surface when deposited at a temperature above T-m and at a critical surface coverage, following a well-established pathway. In contrast, DPPC liposomes larger than 160 nm do not rupture on the surface when adsorbed at 22 degrees C or at 50 degrees C. However, when liposomes of this size are first adsorbed at 22 degrees C and at a high enough surface coverage, after which they are subject to a constant temperature gradient up to 50 degrees C, they rupture and fuse to a bilayer, a process that is initiated around T-m. The results are discussed and interpreted considering a combination of effects derived from liposome-surface and liposome-liposome interactions, different softness/stiffness and shape of liposomes below and above T-m, the dynamics and thermal activation of the bilayers occurring around T-m and (for liposomes containing 33% of NaCl) osmotic pressure. These findings are valuable both for preparation of supported lipid bilayer cell membrane mimics and for designing temperature-responsive material coatings.

MEMBRANES

RUPTURE

SIMULATIONS

THERMOSENSITIVE LIPOSOMES

VESICLE ADSORPTION

PHOSPHOLIPID-BILAYERS

HYPERTHERMIA

DIOXIDE

QCM-D

SURFACE

Författare

Yujia Jing

Chalmers, Teknisk fysik, Biologisk fysik

Chalmers, Signaler och system, Signalbehandling och medicinsk teknik, Medicinska signaler och system

Hana Dobsicek Trefna

Chalmers, Signaler och system, Signalbehandling och medicinsk teknik, Biomedicinsk elektromagnetik

Mikael Persson

Chalmers, Signaler och system, Signalbehandling och medicinsk teknik, Medicinska signaler och system

Bengt Herbert Kasemo

Chalmers, Teknisk fysik, Kemisk fysik

Sofia Svedhem

Chalmers, Teknisk fysik, Biologisk fysik

Soft Matter

1744-683X (ISSN) 1744-6848 (eISSN)

Vol. 10 1 187-195

Ämneskategorier

Materialteknik

DOI

10.1039/c3sm50947h