Electrospun nanofibrous cellulose scaffolds with controlled microarchitecture
Artikel i vetenskaplig tidskrift, 2014

Introducing porosity in electrospun scaffolds is critical to improve cell penetration and nutrient diffusion for tissue engineering. Nanofibrous cellulose scaffolds were prepared by electrospinning cellulose acetate (CA) followed by saponification to regenerate cellulose. Using a computer-assisted design approach, scaffolds underwent laser ablation resulting in pores with diameters between 50 and 300 mu m. without damaging or modifying the surrounding scaffold area. A new mineralization method was employed in conjunction with microablation using commercial phosphate buffered saline (PBS) to soak carboxymethylcellulose surface-modified electrospun scaffolds. The resulting crystals within the scaffold on the interior of the pore had a calcium to phosphate ratio of 1.56, similar to hydroxyapatite. It was observed that porosity of the cellulose scaffolds enhanced osteoblast cell attachment at the edge of the pores, while mineralization enhanced overall cell density.

HYDROXYAPATITE

AFFINITY MEMBRANE

CONSTRUCTS

Porosity

DESIGN

Cellulose

ADHESION

V8

Hydroxyapatite

Laser

ACCOUNTS OF CHEMICAL RESEARCH

PEPTIDES

ARCHITECTURE

Scaffold

Bone regeneration

P273

TISSUE ENGINEERING APPLICATIONS

SURFACE

SNER AS

CALCIUM-PHOSPHATE

1975

Författare

K. Rodriguez

Virginia Polytechnic Institute and State University

Johan Sundberg

Chalmers, Kemi- och bioteknik, Polymerteknologi

Paul Gatenholm

Wallenberg Wood Science Center (WWSC)

Chalmers, Kemi- och bioteknik, Polymerteknologi

S. Renneckar

Virginia Polytechnic Institute and State University

Carbohydrate Polymers

0144-8617 (ISSN)

Vol. 100 143-149

Ämneskategorier

Polymerteknologi

DOI

10.1016/j.carbpol.2012.12.037