On-chip light sheet illumination enables diagnostic size and concentration measurements of membrane vesicles in biofluids
Artikel i vetenskaplig tidskrift, 2014
Cell-derived membrane vesicles that are released in biofluids, like blood or saliva, are emerging as potential non-invasive biomarkers for diseases, such as cancer. Techniques capable of measuring the size and concentration of membrane vesicles directly in biofluids are urgently needed. Fluorescence single particle tracking microscopy has the potential of doing exactly that by labelling the membrane vesicles with a fluorescent label and analysing their Brownian motion in the biofluid. However, an unbound dye in the biofluid can cause high background intensity that strongly biases the fluorescence single particle tracking size and concentration measurements. While such background intensity can be avoided with light sheet illumination, current set-ups require specialty sample holders that are not compatible with high-throughput diagnostics. Here, a microfluidic chip with integrated light sheet illumination is reported, and accurate fluorescence single particle tracking size and concentration measurements of membrane vesicles in cell culture medium and in interstitial fluid collected from primary human breast tumours are demonstrated.
Författare
Hendrik Deschout
Universiteit Gent
K. Raemdonck
Universiteit Gent
S. Stremersch
Universiteit Gent
P. Maoddi
Ecole Polytechnique Federale de Lausanne (EPFL)
G. Mernier
Ecole Polytechnique Federale de Lausanne (EPFL)
P. Renaud
Ecole Polytechnique Federale de Lausanne (EPFL)
S. Jiguet
Ecole Polytechnique Federale de Lausanne (EPFL)
A. Hendrix
Center for Medical Genetics
M. Bracke
Center for Medical Genetics
R. Van den Broecke
Center for Medical Genetics
Magnus Röding
Chalmers, Matematiska vetenskaper, Matematisk statistik
Göteborgs universitet
SuMo Biomaterials
Mats Rudemo
Göteborgs universitet
Chalmers, Matematiska vetenskaper, Matematisk statistik
J. Demeester
Universiteit Gent
S. C. De Smedt
Universiteit Gent
F. Strubbe
Universiteit Gent
K. Neyts
Universiteit Gent
Kevin Braeckmans
Universiteit Gent
Nanoscale
2040-3364 (ISSN) 2040-3372 (eISSN)
Vol. 6 3 1741-1747Ämneskategorier
Kemi
DOI
10.1039/c3nr04432g