On-chip light sheet illumination enables diagnostic size and concentration measurements of membrane vesicles in biofluids
Journal article, 2014
Cell-derived membrane vesicles that are released in biofluids, like blood or saliva, are emerging as potential non-invasive biomarkers for diseases, such as cancer. Techniques capable of measuring the size and concentration of membrane vesicles directly in biofluids are urgently needed. Fluorescence single particle tracking microscopy has the potential of doing exactly that by labelling the membrane vesicles with a fluorescent label and analysing their Brownian motion in the biofluid. However, an unbound dye in the biofluid can cause high background intensity that strongly biases the fluorescence single particle tracking size and concentration measurements. While such background intensity can be avoided with light sheet illumination, current set-ups require specialty sample holders that are not compatible with high-throughput diagnostics. Here, a microfluidic chip with integrated light sheet illumination is reported, and accurate fluorescence single particle tracking size and concentration measurements of membrane vesicles in cell culture medium and in interstitial fluid collected from primary human breast tumours are demonstrated.
Author
Hendrik Deschout
Ghent university
K. Raemdonck
Ghent university
S. Stremersch
Ghent university
P. Maoddi
Swiss Federal Institute of Technology in Lausanne (EPFL)
G. Mernier
Swiss Federal Institute of Technology in Lausanne (EPFL)
P. Renaud
Swiss Federal Institute of Technology in Lausanne (EPFL)
S. Jiguet
Swiss Federal Institute of Technology in Lausanne (EPFL)
A. Hendrix
Center for Medical Genetics
M. Bracke
Center for Medical Genetics
R. Van den Broecke
Center for Medical Genetics
Magnus Röding
Chalmers, Mathematical Sciences, Mathematical Statistics
University of Gothenburg
SuMo Biomaterials
Mats Rudemo
University of Gothenburg
Chalmers, Mathematical Sciences, Mathematical Statistics
J. Demeester
Ghent university
S. C. De Smedt
Ghent university
F. Strubbe
Ghent university
K. Neyts
Ghent university
Kevin Braeckmans
Ghent university
Nanoscale
2040-3364 (ISSN)
Vol. 6 3 1741-1747Subject Categories
Chemical Sciences
DOI
10.1039/c3nr04432g