On-chip light sheet illumination enables diagnostic size and concentration measurements of membrane vesicles in biofluids
Journal article, 2014

Cell-derived membrane vesicles that are released in biofluids, like blood or saliva, are emerging as potential non-invasive biomarkers for diseases, such as cancer. Techniques capable of measuring the size and concentration of membrane vesicles directly in biofluids are urgently needed. Fluorescence single particle tracking microscopy has the potential of doing exactly that by labelling the membrane vesicles with a fluorescent label and analysing their Brownian motion in the biofluid. However, an unbound dye in the biofluid can cause high background intensity that strongly biases the fluorescence single particle tracking size and concentration measurements. While such background intensity can be avoided with light sheet illumination, current set-ups require specialty sample holders that are not compatible with high-throughput diagnostics. Here, a microfluidic chip with integrated light sheet illumination is reported, and accurate fluorescence single particle tracking size and concentration measurements of membrane vesicles in cell culture medium and in interstitial fluid collected from primary human breast tumours are demonstrated.

Author

Hendrik Deschout

Ghent university

K. Raemdonck

Ghent university

S. Stremersch

Ghent university

P. Maoddi

Ecole Polytechnique Federale De Lausanne

G. Mernier

Ecole Polytechnique Federale De Lausanne

P. Renaud

Ecole Polytechnique Federale De Lausanne

S. Jiguet

Ecole Polytechnique Federale De Lausanne

A. Hendrix

Center for Medical Genetics

M. Bracke

Center for Medical Genetics

R. Van den Broecke

Center for Medical Genetics

Magnus Röding

University of Gothenburg

Chalmers, Mathematical Sciences, Mathematical Statistics

SuMo Biomaterials

Mats Rudemo

University of Gothenburg

Chalmers, Mathematical Sciences, Mathematical Statistics

J. Demeester

Ghent university

S. C. De Smedt

Ghent university

F. Strubbe

Ghent university

K. Neyts

Ghent university

Kevin Braeckmans

Ghent university

Nanoscale

2040-3364 (ISSN)

Vol. 6 3 1741-1747

Subject Categories

Chemical Sciences

DOI

10.1039/c3nr04432g

More information

Latest update

5/3/2018 1