Monitoring the osmotic response of single yeast cells through force measurement in the environmental scanning electron microscope
Artikel i vetenskaplig tidskrift, 2014

We present a measurement system that combines an environmental scanning electron microscope (ESEM) and an atomic force microscope (AFM). This combination enables studies of static and dynamic mechanical properties of hydrated specimens, such as individual living cells. The integrated AFM sensor provides direct and continuous force measurement based on piezoresistive force transduction, allowing the recording of events in the millisecond range. The in situ ESEM-AFM setup was used to study Pichia pastoris wild-type yeast cells. For the first time, a quantified measure of the osmotic response of an individual yeast cell inside an ESEM is presented. With this technique, cell size changes due to humidity variations can be monitored with nanometre accuracy. In addition, mechanical properties were extracted from load-displacement curves. A Young's modulus of 13-15 MPa was obtained for the P. pastoris yeast cells. The developed method is highly interesting as a complementary tool for the screening of drugs directed towards cellular water transport activity and provides new possibilities of studying mechanosensitive regulation of aquaporins.

single cell characterization

osmotic response

atomic force microscopy

cell viability

yeast cell

in situ

environmental scanning electron microscopy

Författare

Anna Olsson

SuMo Biomaterials

Chalmers, Teknisk fysik, Eva Olsson Group

Alexandra Nafari

Chalmers, Mikroteknologi och nanovetenskap (MC2)

Kristina Hedfalk

Göteborgs universitet

Eva Olsson

Chalmers, Teknisk fysik, Eva Olsson Group

Krister Svensson

Karlstads universitet

Anke Sanz-Velasco

Chalmers, Teknisk fysik, Elektronikmaterial och system

Measurement Science and Technology

0957-0233 (ISSN) 1361-6501 (eISSN)

Vol. 25 2 Art. no. 025901-

Ämneskategorier

Fysik

DOI

10.1088/0957-0233/25/2/025901

Mer information

Senast uppdaterat

2018-05-23