Monitoring the osmotic response of single yeast cells through force measurement in the environmental scanning electron microscope
Journal article, 2014

We present a measurement system that combines an environmental scanning electron microscope (ESEM) and an atomic force microscope (AFM). This combination enables studies of static and dynamic mechanical properties of hydrated specimens, such as individual living cells. The integrated AFM sensor provides direct and continuous force measurement based on piezoresistive force transduction, allowing the recording of events in the millisecond range. The in situ ESEM-AFM setup was used to study Pichia pastoris wild-type yeast cells. For the first time, a quantified measure of the osmotic response of an individual yeast cell inside an ESEM is presented. With this technique, cell size changes due to humidity variations can be monitored with nanometre accuracy. In addition, mechanical properties were extracted from load-displacement curves. A Young's modulus of 13-15 MPa was obtained for the P. pastoris yeast cells. The developed method is highly interesting as a complementary tool for the screening of drugs directed towards cellular water transport activity and provides new possibilities of studying mechanosensitive regulation of aquaporins.

atomic force microscopy

osmotic response

single cell characterization

yeast cell

in situ

environmental scanning electron microscopy

cell viability

Author

Anna Jansson

SuMo Biomaterials

Chalmers, Applied Physics, Eva Olsson Group

Alexandra Nafari

Chalmers, Microtechnology and Nanoscience (MC2)

Kristina Hedfalk

University of Gothenburg

Eva Olsson

Chalmers, Applied Physics, Eva Olsson Group

Krister Svensson

Karlstad University

Anke Sanz-Velasco

Chalmers, Applied Physics, Electronics Material and Systems Laboratory

Measurement Science and Technology

0957-0233 (ISSN) 1361-6501 (eISSN)

Vol. 25 2 Art. no. 025901-

Subject Categories

Physical Sciences

DOI

10.1088/0957-0233/25/2/025901

More information

Latest update

11/5/2018