Stem cell responses to plasma surface modified electrospun polyurethane scaffolds.
Artikel i vetenskaplig tidskrift, 2014

The topographical effects from functional materials on stem cell behavior are currently of interest in tissue engineering and regenerative medicine. Here we investigate the influence of argon, oxygen, and hydrogen plasma surface modification of electrospun polyurethane fibers on human embryonic stem cell (hESC) and rat postnatal neural stem cell (NSC) responses. The plasma gases were found to induce three combinations of fiber surface functionalities and roughness textures. On randomly oriented fibers, plasma treatments lead to substantially increased hESC attachment and proliferation as compared to native fibers. Argon plasma was found to induce the most optimal combination of surface functionality and roughness for cell expansion. Contact guided migration of cells and alignment of cell processes were observed on aligned fibers. Neuronal differentiation around 5% was found for all samples and was not significantly affected by the induced variations of surface functional group distribution or individual fiber topography.

Polyurethane

Stem cell

Scaffold

Surface modification

Författare

Carl Zandén

Chalmers, Mikroteknologi och nanovetenskap (MC2), Elektronikmaterial och system

Nina Hellström Erkenstam

Göteborgs universitet

Thomas Padel

Göteborgs universitet

Julia Wittgenstein

Göteborgs universitet

Johan Liu

Chalmers, Mikroteknologi och nanovetenskap (MC2), Elektronikmaterial och system

Hans-Georg Kuhn

Göteborgs universitet

Nanomedicine: Nanotechnology, Biology, and Medicine

1549-9634 (ISSN) 1549-9642 (eISSN)

Vol. 10 5 949-958

Ämneskategorier

Neurovetenskaper

Biomaterialvetenskap

DOI

10.1016/j.nano.2014.01.010

PubMed

24524929