Chemical imaging of lignocellulosic biomass by CARS microscopy
Artikel i vetenskaplig tidskrift, 2014

Chemical and structural composition of wood biomass is studied by label-free and chemically specific Coherent Anti-Stokes Raman Scattering (CARS) microscopy. A concept developed for assignment and semi-quantitative imaging of sample components; cellulose, hemicellulose, and lignin; by multiplex CARS microspectroscopy and subsequent data analysis is presented. Specific imaging without fluorescence backround is achieved an order of magnitude faster compared with conventional Raman microscopy. Laser polarization control yield information on molecular arrangement in wood fibers. Narrowband CARS excitation of single vibrations allows for three-dimensional volume imaging. Thus, CARS microscopy has potential as an important instrument for characterization of lignocellulosic materials.

cellulose

wood

lignin

biomass

CARS microscopy

xylan

Författare

C. Pohling

Universität Heidelberg

Christian Brackmann

Chalmers, Kemi- och bioteknik, Molekylär mikroskopi

A. Duarte

Universität Heidelberg

T. Buckup

Universität Heidelberg

Annika Enejder

Chalmers, Kemi- och bioteknik, Molekylär mikroskopi

M. Motzkus

Universität Heidelberg

Journal of Biophotonics

1864-063X (ISSN)

Vol. 7 126-134

Ämneskategorier

Fysikalisk kemi

DOI

10.1002/jbio.201300052