Detoxification of acid pretreated spruce hydrolysates with ferrous sulfate and hydrogen peroxide improves enzymatic hydrolysis and fermentation
Artikel i vetenskaplig tidskrift, 2014

The aim of the present work was to investigate whether a detoxification method already in use during waste water treatment could be functional also for ethanol production based on lignocellulosic substrates. Chemical conditioning of spruce hydrolysate with hydrogen peroxide (H2O2) and ferrous sulfate (FeSO4) was shown to be an efficient strategy to remove significant amounts of inhibitory compounds and, simultaneously, to enhance the enzymatic hydrolysis and fermentability of the substrates. Without treatment, the hydrolysates were hardly fermentable with maximum ethanol concentration below 0.4 g/l. In contrast, treatment by 2.5 mM FeSO4 and 150 mM H2O2 yielded a maximum ethanol concentration of 8.3 g/l.


V. P. Soudham

Umeå universitet

Chalmers, Kemi- och bioteknik, Livsvetenskaper

Tomas Brandberg

Högskolan i Borås

J. P. Mikkola

Umeå universitet

Åbo Akademi

Christer Larsson

Chalmers, Kemi- och bioteknik, Livsvetenskaper

Bioresource Technology

0960-8524 (ISSN)

Vol. 166 559-565





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