Nanometer Scale Titanium Surface Texturing Are Detected by Signaling Pathways Involving Transient FAK and Src Activations
Artikel i vetenskaplig tidskrift, 2014

Background: It is known that physico/chemical alterations on biomaterial surfaces have the capability to modulate cellular behavior, affecting early tissue repair. Such surface modifications are aimed to improve early healing response and, clinically, offer the possibility to shorten the time from implant placement to functional loading. Since FAK and Src are intracellular proteins able to predict the quality of osteoblast adhesion, this study evaluated the osteoblast behavior in response to nanometer scale titanium surface texturing by monitoring FAK and Src phosphorylations. Methodology: Four engineered titanium surfaces were used for the study: machined (M), dual acid-etched (DAA), resorbable media microblasted and acid-etched (MBAA), and acid-etch microblasted (AAMB). Surfaces were characterized by scanning electron microscopy, interferometry, atomic force microscopy, x-ray photoelectron spectroscopy and energy dispersive X-ray spectroscopy. Thereafter, those 4 samples were used to evaluate their cytotoxicity and interference on FAK and Src phosphorylations. Both Src and FAK were investigated by using specific antibody against specific phosphorylation sites. Principal Findings: The results showed that both FAK and Src activations were differently modulated as a function of titanium surfaces physico/chemical configuration and protein adsorption. Conclusions: It can be suggested that signaling pathways involving both FAK and Src could provide biomarkers to predict osteoblast adhesion onto different surfaces.


W. F. Zambuzzi

UNESP-Universidade Estadual Paulista

E. A. Bonfante

Universidade de Sao Paulo - USP

Ryo Jimbo

Malmö universitet

Mariko Hayashi

Malmö universitet

Martin Andersson

Chalmers, Kemi- och bioteknik, Teknisk ytkemi

G. Alves

Universidade Federal Fluminense

E. R. Takamori

Excellion Biomedical Services

P. J. Beltrao

Instituto Nacional de Metrologia

Paulo G Coelho

NYU College of Dentistry

J. M. Granjeiro

Instituto Nacional de Metrologia


1932-6203 (ISSN)

Vol. 9 e95662