Identification and qualitative characterization of high and low lignin lines from an oat TILLING population
Artikel i vetenskaplig tidskrift, 2014

To identify differences in seed lignin content, 520 randomly chosen independent lines were screened in mutagenized oat population and lines with the seed lignin levels ranging from 20 to 63 gkg(-1) were identified. In the commercial variety Belinda, from which the mutated population was developed, seed lignin level was determined to be 41 g kg(-1). In Assiniboia, a Canadian low lignin variety, it was found to be 21 g kg(-1). To investigate if extracted lignin from the mutated lines were also qualitatively different from Belinda, two lines with the lowest and highest lignin levels were selected for structural analyses using XRD, UV and FT-IR spectroscopy. Results showed that there were significant qualitative differences in seed lignin in the mutated lines and in Belinda, and based on this, we predict that lignin from the mutated seeds will be more digestible in ruminant animals than Belinda seeds. This prediction was confirmed in preliminary in vitro digestion experiments.

Belinda

FT-IR spectroscopy

Dioxane

UV

Lignin

Oat

Assiniboia

Författare

Vivekanand Vivekanand

Göteborgs universitet

Aakash Chawade

Lunds universitet

Mikael Larsson

SuMo Biomaterials

Chalmers, Kemi- och bioteknik, Farmaceutisk teknologi

Anette Larsson

SuMo Biomaterials

Chalmers, Kemi- och bioteknik, Farmaceutisk teknologi

Olof Olsson

Lunds universitet

Industrial Crops and Products

0926-6690 (ISSN)

Vol. 59 1-8

Ämneskategorier

Biokemi och molekylärbiologi

DOI

10.1016/j.indcrop.2014.04.019