Construction and Modeling of Concatemeric DNA Multilayers on a Planar Surface as Monitored by QCM-D and SPR
Artikel i vetenskaplig tidskrift, 2014

The sequential hybridization of a 534 base pair DNA concatemer layer was monitored by QCM-D and SPR, and the QCM-D data were analyzed by Voigt viscoelastic models. The results show that Voigt-based modeling gives a good description of the experimental data but only if shear viscosity and elasticity are allowed to depend on the shear frequency. The derived layer thickness, shear viscosity and elasticity of the growing film give a representation of the DNA film in agreement with known bulk properties of DNA, and reveal a maximum in film viscosity when the molecules in the layer contain 75 base pairs. The experimental data during construction of a 3084 bp DNA concatemer layer were compared to predictions of the QCM-D response of a 1 mu m thick film of rod-like polymers. A predicted nonmonotonous variation of dissipation with frequency (added mass) is in qualitative agreement with the experiments, but with a quantitative disagreement which likely reflects that the flexibility of such long DNA molecules is not included in the model.

VISCOELASTIC PROPERTIES

GOLD SURFACES

PLASMON RESONANCE BIOSENSOR

FILMS

IMMOBILIZATION

QUARTZ-CRYSTAL MICROBALANCE

HYBRIDIZATION

MOLECULE

CONFORMATION

SUBSEQUENT

SPECTROSCOPY

Författare

Lu Sun

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

Sofia Svedhem

Chalmers, Teknisk fysik, Biologisk fysik

Björn Åkerman

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

Langmuir

0743-7463 (ISSN) 1520-5827 (eISSN)

Vol. 30 8432-8441

Ämneskategorier

Den kondenserade materiens fysik

DOI

10.1021/la500716d