Disposable microfluidic chip with integrated light sheet illumination enables diagnostics based on membrane vesicles
Paper i proceeding, 2014

Cell-derived membrane vesicles that are released in body fluids are emerging as potential non-invasive biomarkers for diseases like cancer. Techniques capable of measuring the size and concentration of such membrane vesicles directly in body fluids are urgently needed. Here we report on a microfluidic chip with integrated light sheet illumination, and demonstrate accurate fluorescence Single Particle Tracking measurements of the size and concentration of membrane vesicles in cell culture medium and in interstitial fluid collected from primary human breast tumours.

Diagnostics

Light sheet microscopy

Single Particle Tracking

Lab-on-a-chip

Författare

Hendrik Deschout

K. Raemdonck

S. Stremersch

P. Maoddi

G. Mernier

P. Renaud

S. Jiguet

A. Hendrix

M. Bracke

R. Van den Broecke

Magnus Röding

Göteborgs universitet

SuMo Biomaterials

Chalmers, Matematiska vetenskaper, matematisk statistik

Mats Rudemo

Chalmers, Matematiska vetenskaper, matematisk statistik

Göteborgs universitet

J. Demeester

S. De Smedt

F. Strubbe

K. Neyts

Kevin Braeckmans

17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Freiburg; Germany; 27 October 2013 through 31 October 2013

Vol. 3 2010-2012

Ämneskategorier

Biokemi och molekylärbiologi

ISBN

978-16-32-66624-6

Mer information

Skapat

2017-10-08