Disposable microfluidic chip with integrated light sheet illumination enables diagnostics based on membrane vesicles
Paper i proceeding, 2013

Cell-derived membrane vesicles that are released in body fluids are emerging as potential non-invasive biomarkers for diseases like cancer. Techniques capable of measuring the size and concentration of such membrane vesicles directly in body fluids are urgently needed. Here we report on a microfluidic chip with integrated light sheet illumination, and demonstrate accurate fluorescence Single Particle Tracking measurements of the size and concentration of membrane vesicles in cell culture medium and in interstitial fluid collected from primary human breast tumours.

Diagnostics

Single Particle Tracking

Light sheet microscopy

Lab-on-a-chip

Författare

Hendrik Deschout

Universiteit Gent

K. Raemdonck

Universiteit Gent

S. Stremersch

Universiteit Gent

P. Maoddi

Ecole Polytechnique Federale De Lausanne

G. Mernier

Ecole Polytechnique Federale De Lausanne

P. Renaud

Ecole Polytechnique Federale De Lausanne

S. Jiguet

Ecole Polytechnique Federale De Lausanne

A. Hendrix

Universiteit Gent

M. Bracke

Universiteit Gent

R. Van den Broecke

Universiteit Gent

Magnus Röding

Chalmers, Matematiska vetenskaper, Tillämpad matematik och statistik

Göteborgs universitet

Mats Rudemo

Göteborgs universitet

Chalmers, Matematiska vetenskaper, Tillämpad matematik och statistik

J. Demeester

Universiteit Gent

S. De Smedt

Universiteit Gent

F. Strubbe

Universiteit Gent

K. Neyts

Universiteit Gent

Kevin Braeckmans

Universiteit Gent

17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Freiburg; Germany; 27 October 2013 through 31 October 2013

Vol. 3 2010-2012

Ämneskategorier

Biokemi och molekylärbiologi

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Senast uppdaterat

2019-11-26