Disposable microfluidic chip with integrated light sheet illumination enables diagnostics based on membrane vesicles
Paper in proceedings, 2013

Cell-derived membrane vesicles that are released in body fluids are emerging as potential non-invasive biomarkers for diseases like cancer. Techniques capable of measuring the size and concentration of such membrane vesicles directly in body fluids are urgently needed. Here we report on a microfluidic chip with integrated light sheet illumination, and demonstrate accurate fluorescence Single Particle Tracking measurements of the size and concentration of membrane vesicles in cell culture medium and in interstitial fluid collected from primary human breast tumours.

Diagnostics

Single Particle Tracking

Light sheet microscopy

Lab-on-a-chip

Author

Hendrik Deschout

Ghent university

K. Raemdonck

Ghent university

S. Stremersch

Ghent university

P. Maoddi

Ecole Polytechnique Federale De Lausanne

G. Mernier

Ecole Polytechnique Federale De Lausanne

P. Renaud

Ecole Polytechnique Federale De Lausanne

S. Jiguet

Ecole Polytechnique Federale De Lausanne

A. Hendrix

Ghent university

M. Bracke

Ghent university

R. Van den Broecke

Ghent university

Magnus Röding

Chalmers, Mathematical Sciences, Applied Mathematics and Statistics

University of Gothenburg

Mats Rudemo

University of Gothenburg

Chalmers, Mathematical Sciences, Applied Mathematics and Statistics

J. Demeester

Ghent university

S. De Smedt

Ghent university

F. Strubbe

Ghent university

K. Neyts

Ghent university

Kevin Braeckmans

Ghent university

17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Freiburg; Germany; 27 October 2013 through 31 October 2013

Vol. 3 2010-2012

Subject Categories

Biochemistry and Molecular Biology

More information

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11/26/2019