Disposable microfluidic chip with integrated light sheet illumination enables diagnostics based on membrane vesicles
Paper in proceeding, 2013
Cell-derived membrane vesicles that are released in body fluids are emerging as potential non-invasive biomarkers for diseases like cancer. Techniques capable of measuring the size and concentration of such membrane vesicles directly in body fluids are urgently needed. Here we report on a microfluidic chip with integrated light sheet illumination, and demonstrate accurate fluorescence Single Particle Tracking measurements of the size and concentration of membrane vesicles in cell culture medium and in interstitial fluid collected from primary human breast tumours.
Diagnostics
Single Particle Tracking
Light sheet microscopy
Lab-on-a-chip
Author
Hendrik Deschout
Ghent university
K. Raemdonck
Ghent university
S. Stremersch
Ghent university
P. Maoddi
Swiss Federal Institute of Technology in Lausanne (EPFL)
G. Mernier
Swiss Federal Institute of Technology in Lausanne (EPFL)
P. Renaud
Swiss Federal Institute of Technology in Lausanne (EPFL)
S. Jiguet
Swiss Federal Institute of Technology in Lausanne (EPFL)
A. Hendrix
Ghent university
M. Bracke
Ghent university
R. Van den Broecke
Ghent university
Magnus Röding
Chalmers, Mathematical Sciences, Applied Mathematics and Statistics
University of Gothenburg
Mats Rudemo
University of Gothenburg
Chalmers, Mathematical Sciences, Applied Mathematics and Statistics
J. Demeester
Ghent university
S. De Smedt
Ghent university
F. Strubbe
Ghent university
K. Neyts
Ghent university
Kevin Braeckmans
Ghent university
17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Freiburg; Germany; 27 October 2013 through 31 October 2013
Vol. 3 2010-2012
Subject Categories
Biochemistry and Molecular Biology