Disposable microfluidic chip with integrated light sheet illumination enables diagnostics based on membrane vesicles
Paper in proceedings, 2014

Cell-derived membrane vesicles that are released in body fluids are emerging as potential non-invasive biomarkers for diseases like cancer. Techniques capable of measuring the size and concentration of such membrane vesicles directly in body fluids are urgently needed. Here we report on a microfluidic chip with integrated light sheet illumination, and demonstrate accurate fluorescence Single Particle Tracking measurements of the size and concentration of membrane vesicles in cell culture medium and in interstitial fluid collected from primary human breast tumours.

Diagnostics

Light sheet microscopy

Single Particle Tracking

Lab-on-a-chip

Author

Hendrik Deschout

K. Raemdonck

S. Stremersch

P. Maoddi

G. Mernier

P. Renaud

S. Jiguet

A. Hendrix

M. Bracke

R. Van den Broecke

Magnus Röding

University of Gothenburg

SuMo Biomaterials

Chalmers, Mathematical Sciences, Mathematical Statistics

Mats Rudemo

Chalmers, Mathematical Sciences, Mathematical Statistics

University of Gothenburg

J. Demeester

S. De Smedt

F. Strubbe

K. Neyts

Kevin Braeckmans

17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Freiburg; Germany; 27 October 2013 through 31 October 2013

Vol. 3 2010-2012

Subject Categories

Biochemistry and Molecular Biology

ISBN

978-16-32-66624-6

More information

Created

10/8/2017