DNA-Binding Properties of Amidine-Substituted Spiropyran Photoswitches
Artikel i vetenskaplig tidskrift, 2014

Two amidine-substituted spiropyran derivatives have been characterized with respect to the DNA-binding properties over a broad pH interval. The two derivatives differ in the number of positive charges. By varying the pH, the protonation state of the derivatives is also changed, allowing for additional variations in the charge distribution. We show that the closed spiro isomer does not bind for either of the two derivatives, whereas the open merocyanine forms bind both in the protonated and in the nonprotonated state, but with dramatically different binding constants. Flow-oriented linear dichroism (LD) measurements also show that there are differences in the binding modes between the various forms. We rationalize these differences in terms of structure and charge distribution.

photochromism

DNA-binding

DNA

spiropyrans

photoswitches

Författare

Martin Hammarson

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

Jesper Nilsson

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

Shiming Li

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

Per Lincoln

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

Joakim Andreasson

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

Chemistry - A European Journal

0947-6539 (ISSN) 1521-3765 (eISSN)

Vol. 20 48 15855-15862

Photochromic Systems for Solid State Molecular Electronic Devices and Light-Activated Cancer Drugs (PHOTOCHROMES)

Europeiska kommissionen (FP7), 2008-09-01 -- 2013-08-31.

Ämneskategorier

Kemiteknik

Annan medicinsk grundvetenskap

DOI

10.1002/chem.201405113