Increase of Compact Bone Thickness in Rat Tibia after Implanting MgO into the Bone Marrow Cavity
Artikel i vetenskaplig tidskrift, 2014

The effect of implanting MgO paste into the bone marrow of rat tibia, was studied by light microscopy, time of flight-secondary ion mass spectrometry (ToF-SIMS), and environmental scanning electron microscopy (ESEM), and energy dispersive X-ray (EDX) analysis. After three weeks of implantation, the thickness of compact bone increased by 25% compared to sham-operated controls, while no effect was seen on the trabecular bone. In order to further elucidate the mechanism of the Mg-induced increase in bone mass, EDX and ToF-SIMS analysis of the bone samples was made at two weeks. At this time-point, no detectable difference in the thickness of the compact bone in Mg-treated and non-treated animals was observed. The Mg-content of the bone marrow and bone tissue of the Mg-exposed animals did not differ from that of sham-operated controls, implying that there are no traces of the implanted MgO when the mass of compact bone increases, between two and three weeks after surgery. The ratio of Mg/Ca content was higher in the bone of Mg-treated animals, indicating an altered structure of the bone mineral, which was confirmed by the ToF-SIMS analysis, showing increased levels of MgCO3, phosphate ions and CaF in the bone of MgO-exposed animals. Possible cellular activities behind the effect of MgO on compact bone thickness are discussed.

rat model

bone remodeling

bone density

bone healing

magnesium-oxide

ToF-SIMS

EDX

Författare

Håkan Nygren

Göteborgs universitet

Mobina Chaudhry

Göteborgs universitet

Stefan Gustafsson

Chalmers, Teknisk fysik

SuMo Biomaterials

Göran Kjeller

Göteborgs universitet

Per Malmberg

Chalmers, Kemi- och bioteknik, Analytisk kemi

K-E. Johansson

Journal of Functional Biomaterials

2079-4983 (eISSN)

Vol. 5 3 158-166

DOI

10.3390/jfb5030158

Mer information

Skapat

2017-10-07