Droplet based directed evolution of yeast cell factories doubles production of industrial enzymes
Paper i proceeding, 2013

We present a high throughput micro-droplet based method for directed evolution of yeast cell factories for improved production of industrial enzymes. The workflow includes a fluorescently activated droplet sorter which was found to accurately sort droplets with a false positive rate of 0.0002 at 300 Hz. The workflow was used to screen a library of α-amylase expressing yeast mutants. A candidate yeast strain with a more than twofold increase in α-amylase production was isolated from a single round of directed evolution.

Författare

S. L. Sjostrom

Kungliga Tekniska Högskolan (KTH)

Y. Bai

Kungliga Tekniska Högskolan (KTH)

M. Huang

Chalmers University of Technology

Jens B Nielsen

Kemi- och bioteknik, Livsvetenskaper, Systembiologi

H. N. Joensson

Kungliga Tekniska Högskolan (KTH)

H. A. Svahn

Kungliga Tekniska Högskolan (KTH)

17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013

1270-1272

Ämneskategorier

Industriell bioteknik

ISBN

978-163266624-6