Droplet based directed evolution of yeast cell factories doubles production of industrial enzymes
Paper in proceedings, 2013

We present a high throughput micro-droplet based method for directed evolution of yeast cell factories for improved production of industrial enzymes. The workflow includes a fluorescently activated droplet sorter which was found to accurately sort droplets with a false positive rate of 0.0002 at 300 Hz. The workflow was used to screen a library of α-amylase expressing yeast mutants. A candidate yeast strain with a more than twofold increase in α-amylase production was isolated from a single round of directed evolution.

Author

S. L. Sjostrom

Royal Institute of Technology (KTH)

Y. Bai

Royal Institute of Technology (KTH)

Mingtao Huang

Chalmers, Chemical and Biological Engineering, Life Sciences, System Biology

Jens B Nielsen

Royal Institute of Technology (KTH)

Novo Nordisk Foundation Center for Biosustainability

Chalmers, Chemical and Biological Engineering, Life Sciences, System Biology

H. N. Joensson

Royal Institute of Technology (KTH)

H. A. Svahn

Royal Institute of Technology (KTH)

17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013

1270-1272

Subject Categories

Industrial Biotechnology

ISBN

978-163266624-6

More information

Latest update

12/27/2018