Secretion of non-cell-bound phytase by the yeast Pichia kudriavzevii TY13
Artikel i vetenskaplig tidskrift, 2015

AimsMineral deficiencies cause several health problems in the world, especially for populations consuming cereal-based diets rich in the anti-nutrient phytate. Our aim was to characterize the phytate-degrading capacity of the yeast Pichia kudriavzevii TY13 and its secretion of phytase. Methods and ResultsThe phytase activity in cell-free supernatants from cultures with 100% intact cells was 35-190mUml(-1) depending on the media. The K-m was 028mmoll(-1) and the specific phytase activity 032Umg(-1) total protein. The phytase activity and secretion of extracellular non-cell-bound phytase was affected by the medium phosphate concentrations. Further, addition of yeast extract had a clearly inducing effect, resulting in over 60% of the cultures total phytase activity as non-cell-bound. ConclusionsOur study reveals that it is possible to achieve high extracellular phytase activity from the yeast P.kudriavzevii TY13 by proper composition of the growth medium. Significance and Impact of the StudyTY13 could be a promising future starter culture for fermented foods with improved mineral bioavailability. Using strains that secrete phytase to the food matrix may significantly improve the phytate degradation by facilitating the enzyme-to-substrate interaction. The secreted non-cell-bound phytase activities by TY13 could further be advantageous in industrial production of phytase.

food fermentation

yeast

phytate

Pichia kudriavzevii

phytase

extracellular

Författare

[Person 6262401f-9218-48db-a756-65401f2648dc not found]

Chalmers, Biologi och bioteknik, Livsmedelsvetenskap

[Person fec62cec-b278-4745-ba60-07ceeca528c9 not found]

Chalmers, Biologi och bioteknik, Livsmedelsvetenskap

[Person 66c3ded2-a16d-4746-b68d-895cefecaf5f not found]

Chalmers, Biologi och bioteknik, Livsmedelsvetenskap

[Person f04cd091-f905-4c5c-9e31-c5439db66647 not found]

Chalmers, Biologi och bioteknik, Industriell bioteknik

[Person 0f87a827-ee6a-426a-8c82-5784bb03295a not found]

Chalmers, Biologi och bioteknik, Livsmedelsvetenskap

Journal of Applied Microbiology

1364-5072 (ISSN) 1365-2672 (eISSN)

Vol. 118 5 1126-1136

Ämneskategorier

Livsmedelsteknik

Styrkeområden

Livsvetenskaper och teknik (2010-2018)

DOI

10.1111/jam.12767

Mer information

Skapat

2017-10-07