The human cardiac and skeletal muscle proteomes defined by transcriptomics and antibody-based profiling
Artikel i vetenskaplig tidskrift, 2015

Background: To understand cardiac and skeletal muscle function, it is important to define and explore their molecular constituents and also to identify similarities and differences in the gene expression in these two different striated muscle tissues. Here, we have investigated the genes and proteins with elevated expression in cardiac and skeletal muscle in relation to all other major human tissues and organs using a global transcriptomics analysis complemented with antibody-based profiling to localize the corresponding proteins on a single cell level. Results: Our study identified a comprehensive list of genes expressed in cardiac and skeletal muscle. The genes with elevated expression were further stratified according to their global expression pattern across the human body as well as their precise localization in the muscle tissues. The functions of the proteins encoded by the elevated genes are well in line with the physiological functions of cardiac and skeletal muscle, such as contraction, ion transport, regulation of membrane potential and actomyosin structure organization. A large fraction of the transcripts in both cardiac and skeletal muscle correspond to mitochondrial proteins involved in energy metabolism, which demonstrates the extreme specialization of these muscle tissues to provide energy for contraction. Conclusions: Our results provide a comprehensive list of genes and proteins elevated in striated muscles. A number of proteins not previously characterized in cardiac and skeletal muscle were identified and localized to specific cellular subcompartments. These proteins represent an interesting starting point for further functional analysis of their role in muscle biology and disease.

Transcriptome

Proteome

Cardiac and skeletal muscle

Författare

C. Lindskog

Uppsala universitet

J. Linne

Uppsala universitet

L. Fagerberg

AlbaNova University Center

B. M. Hallstrom

AlbaNova University Center

C. J. Sundberg

Karolinska Institutet

M. Lindholm

Karolinska Institutet

M. Huss

Stockholms universitet

C. Kampf

Uppsala universitet

H. Choi

David Geffen School of Medicine at UCLA

D. A. Liem

David Geffen School of Medicine at UCLA

P. P. Ping

David Geffen School of Medicine at UCLA

Leif Wigge

Chalmers, Biologi och bioteknik, Systembiologi

Adil Mardinoglu

Chalmers, Biologi och bioteknik, Systembiologi

Jens B Nielsen

Chalmers, Biologi och bioteknik, Systembiologi

E. G. Larsson

University of Uppsala Rudbeck Laboratory

F. Ponten

Uppsala universitet

M. Uhlen

AlbaNova University Center

David Geffen School of Medicine at UCLA

BMC Genomics

1471-2164 (ISSN)

Vol. 16 475

Styrkeområden

Livsvetenskaper och teknik

Ämneskategorier

Genetik och förädling

DOI

10.1186/s12864-015-1686-y

PubMed

26109061