Osteogenic potential of human adipose-derived stromal cells on 3-dimensional mesoporous TiO2 coating with magnesium impregnation
Artikel i vetenskaplig tidskrift, 2015

The aim of this study was to evaluate the osteogenic response of human adipose-derived stromal cells (ADScs) to mesoporous titania (TiO2) coatings produced with evaporation-induced self-assembly method (EISA) and loaded with magnesium. Our emphasis with the magnesium release functionality was to modulate progenitor cell osteogenic differentiation under standard culture conditions. Osteogenic properties of the coatings were assessed for stromal cells by means of scanning electron microscopy (SEM) imaging, colorimetric mitochondrial viability assay (MTT), colorimetric alkaline phosphates activity (ALP) assay and real time RT-polymerase chain reaction (PCR). Using atomic force microscopy (AFM) it was shown that the surface expansion area (S-dr) was strongly enhanced by the presence of magnesium. From MTT results it was shown that ADSc viability was significantly increased on mesoporous surfaces compared to the non-porous one at a longer cell culture time. However, no differences were observed between the magnesium impregnated and non-impregnated surfaces. The alkaline phosphatase activity confirmed that ADSc started to differentiate into the osteogenic phenotype after 2 weeks of culturing. The gene expression profile at 2 weeks of cell growth showed that such coatings were capable to incorporate specific osteogenic markers inside their interconnected nano-pores and, at 3 weeks, ADSc differentiated into osteoblasts. Interestingly, magnesium significantly promoted the osteopontin gene expression, which is an essential gene for the early biomaterial-cell osteogenic interaction. (C) 2015 Elsevier B.V. All rights reserved.

Osteogenic markers

Osteogenesis

Magnesium

3-D nanostructure

Osteopontin

Författare

F. Cecchinato

Malmo University Faculty of Odontology

Johan Karlsson

Kemi och kemiteknik, Tillämpad kemi, Teknisk ytkemi

L. Ferroni

Universita degli Studi di Padova

C. Gardin

Universita degli Studi di Padova

S. Galli

Malmo University Faculty of Odontology

A. Wennerberg

Malmo University Faculty of Odontology

B. Zavan

Universita degli Studi di Padova

Martin Andersson

Kemi och kemiteknik, Tillämpad kemi, Teknisk ytkemi

Ryo Jimbo

Nagasaki University

Malmo University Faculty of Odontology

Materials Science and Engineering C

0928-4931 (ISSN)

Vol. 52 225-234

Ämneskategorier

Cellbiologi

Bearbetnings-, yt- och fogningsteknik

DOI

10.1016/j.msec.2015.03.026

PubMed

25953562