Current progress in high cell density yeast bioprocesses for bioethanol production
Artikel i vetenskaplig tidskrift, 2015

High capital costs and low reaction rates are major challenges for establishment of fermentation-based production systems in the bioeconomy. Using high cell density cultures is an efficient way to increase the volumetric productivity of fermentation processes, thereby enabling faster and more robust processes and use of smaller reactors. In this review, we summarize recent progress in the application of high cell density yeast bioprocesses for first and second generation bioethanol production. High biomass concentrations obtained by retention of yeast cells in the reactor enables easier cell reuse, simplified product recovery and higher dilution rates in continuous processes. High local cell density cultures, in the form of encapsulated or strongly flocculating yeast, furthermore obtain increased tolerance to convertible fermentation inhibitors and utilize glucose and other sugars simultaneously, thereby overcoming two additional hurdles for second generation bioethanol production. These effects are caused by local concentration gradients due to diffusion limitations and conversion of inhibitors and sugars by the cells, which lead to low local concentrations of inhibitors and glucose. Quorum sensing may also contribute to the increased stress tolerance. Recent developments indicate that high cell density methodology, with emphasis on high local cell density, offers significant advantages for sustainable second generation bioethanol production.

Biorefinery

Flocculation

Industrial biotechnology

Bioencapsulation

Biofuels

Författare

Johan Westman

Chalmers, Biologi och bioteknik, Industriell bioteknik

Carl Johan Franzén

Chalmers, Biologi och bioteknik, Industriell bioteknik

Biotechnology journal

1860-6768 (ISSN) 1860-7314 (eISSN)

Vol. 10 8 1185-1195

Drivkrafter

Hållbar utveckling

Ämneskategorier

Industriell bioteknik

Bioprocessteknik

Mikrobiologi

Annan industriell bioteknik

Styrkeområden

Energi

Livsvetenskaper och teknik

DOI

10.1002/biot.201400581

PubMed

26211654

Mer information

Skapat

2017-10-08