Visualizing the entire DNA from a chromosome in a single frame
Artikel i vetenskaplig tidskrift, 2015

The contiguity and phase of sequence information are intrinsic to obtain complete understanding of the genome and its relationship to phenotype. We report the fabrication and application of a novel nanochannel design that folds megabase lengths of genomic DNA into a systematic back-and-forth meandering path. Such meandering nanochannels enabled us to visualize the complete 5.7 Mbp (1mm) stained DNA length of a Schizosaccharomyces pombe chromosome in a single frame of a CCD. We were able to hold the DNA in situ while implementing partial denaturation to obtain a barcode pattern that we could match to a reference map using the Poland-Scheraga model for DNA melting. The facility to compose such long linear lengths of genomic DNA in one field of view enabled us to directly visualize a repeat motif, count the repeat unit number, and chart its location in the genome by reference to unique barcode motifs found at measurable distances from the repeat. Meandering nanochannel dimensions can easily be tailored to human chromosome scales, which would enable the whole genome to be visualized in seconds.


Camilla Freitag

Göteborgs universitet

C. Noble

Lunds universitet

Joachim Fritzsche

Göteborgs universitet

Chalmers, Teknisk fysik, Kemisk fysik

Fredrik Persson

Göteborgs universitet

M. Reiter-Schad

Lunds universitet

A. Nilsson

Lunds universitet

Annette Graneli

Göteborgs universitet

T. Ambjornsson

Lunds universitet

K. U. Mir

Wellcome Trust Centre for Human Genetics

Harvard University

J. O. Tegenfeldt

Göteborgs universitet


1932-1058 (ISSN)

Vol. 9 044114- 044114


Biokemi och molekylärbiologi