Supported lipid bilayer repair mediated by AH peptide
Artikel i vetenskaplig tidskrift, 2016

The adsorption and fusion of small unilamellar lipid vesicles on silica-based substrates such as glass is a common method used to fabricate supported lipid bilayers. Successful bilayer formation depends on a number of experimental conditions as well as on the quality of the vesicle preparation. Inevitably, a small fraction of unruptured vesicles always remains in a supported bilayer, and this kind of defect can have devastating influences on the morphological and electrical properties of the supported bilayer when used as a biosensing platform. In this paper, a simple method is reported to improve the completeness of supported bilayers by adding a vesicle rupturing peptide as a final step in the fabrication process. Peptide treatment reduces the fraction of unruptured vesicles to less than 1%, as determined by epifluorescence microscopy and quartz crystal microbalance-dissipation experiments. This step can easily be incorporated into existing procedures for preparing high-quality supported lipid bilayers.

in-situ

alpha-helical peptide

membranes

rupture

quartz-crystal microbalance

unilamellar vesicles

dissipation

vesicle adsorption

phospholipid-bilayers

osmotic-pressure

Författare

M. C. Kim

Nanyang Technological University

Anders Gunnarsson

Chalmers, Fysik, Biologisk fysik

Seyed Tabaei

Nanyang Technological University

Fredrik Höök

Chalmers, Fysik, Biologisk fysik

N. J. Cho

Nanyang Technological University

Physical Chemistry Chemical Physics

1463-9076 (ISSN) 1463-9084 (eISSN)

Vol. 18 3040-3047

Ämneskategorier

Fysik

DOI

10.1039/c5cp06472d