BioH2 photoproduction by means of Rhodopseudomonas palustris sp. cultured in a lab-scale photobioreactor operated in batch, fed-batch and semi-continuous modes
Artikel i vetenskaplig tidskrift, 2015

© 2015 Elsevier Ltd. The present investigation deals with Rhodopseudomonas palustris sp. grown, using acetate, for purposes of hydrogen generation. All experiments were carried out using a lab-scale photobioreactor. The cultures were irradiated with a 150 W (OSRAM power star HQI-TS) lamp impinging on the reactor surface an irradiance of 74 W/m2. Three different growth regimens were compared: batch, fed-batch, and semi-continuous. In the third approach, two daily dilutions were applied, in order to operate the photobioreactor at two hydraulic retention times: 240 h in the first semi-continuous regimen (SC240h) and 60 h in the second (SC60h). The best performances were attained when operating the photobioreactor in fed-batch mode. A maximum hydrogen production rate of 15.21 mL(H2)/L h was achieved. The hydrogen yield (YH) was 1.60 mol H2/mol acetic acid in SC240h; 1.42 mol H2/mol acetic acid when the culture was operated in fed-batch mode, and 0.85 mol H2/mol acetic acid under a batch growth regimen. YH decreased to 0.36 mol H2/mol acetic acid in SC60h. Significant changes in the oxidation-reduction potential were noted among the selected growth regimens tested then and also over time.

Hydrogen yield

Rhodopseudomonas palustris

Hydrogen production rate





G. Padovani

Consiglio Nazionale delle Ricerche

Sigita Vaiciulyte

Chalmers, Biologi och bioteknik, Industriell bioteknik

P. Carlozzi

Consiglio Nazionale delle Ricerche


0016-2361 (ISSN)

Vol. 166 203-210