Production of fatty acid-derived oleochemicals and biofuels by synthetic yeast cell factories
Artikel i vetenskaplig tidskrift, 2016

Sustainable production of oleochemicals requires establishment of cell factory platform strains. The yeast Saccharomyces cerevisiae is an attractive cell factory as new strains can be rapidly implemented into existing infrastructures such as bioethanol production plants. Here we show high-level production of free fatty acids (FFAs) in a yeast cell factory, and the production of alkanes and fatty alcohols from its descendants. The engineered strain produces up to 10.4 g/L of FFAs, which is the highest reported titre to date. Furthermore, through screening of specific pathway enzymes, endogenous alcohol dehydrogenases and aldehyde reductases, we reconstruct efficient pathways for conversion of fatty acids to alkanes (0.8 mg /L) and fatty alcohols (1.5 g/L), to our knowledge the highest titres reported in S. cerevisiae. This should facilitate the construction of yeast cell factories for production of fatty acids derived products and even aldehyde-derived chemicals of high value.

Metabolic engineering

Fatty acid

Yeast cell factories

Olechemicals

Författare

Yongjin Zhou

Chalmers, Biologi och bioteknik, Systembiologi

Nicolaas Buijs

Chalmers, Biologi och bioteknik, Systembiologi

Zhu Zhiwei

Chalmers, Biologi och bioteknik, Systembiologi

Jiufu Qin

Chalmers, Biologi och bioteknik, Systembiologi

Verena Siewers

Chalmers, Biologi och bioteknik, Systembiologi

Jens B Nielsen

Chalmers, Biologi och bioteknik, Systembiologi

Nature Communications

2041-1723 (ISSN)

Vol. 7 11709-

Ämneskategorier

Biokemikalier

Bioenergi

Biokatalys och enzymteknik

Infrastruktur

Chalmers infrastruktur för masspektrometri

Styrkeområden

Energi

Livsvetenskaper och teknik

DOI

10.1038/ncomms11709