The influence of alternative pathways of respiration that utilize branched-chain amino acids following water shortage in Arabidopsis
Artikel i vetenskaplig tidskrift, 2016

During dark-induced senescence isovaleryl-CoA dehydrogenase (IVDH) and D-2-hydroxyglutarate dehydrogenase (D-2HGDH) act as alternate electron donors to the ubiquinol pool via the electron-transfer flavoprotein/electron-transfer flavoprotein:ubiquinone oxidoreductase (ETF/ETFQO) pathway. However, the role of this pathway in response to other stresses still remains unclear. Here, we demonstrated that this alternative pathway is associated with tolerance to drought in Arabidopsis. In comparison with wild type (WT) and lines overexpressing D-2GHDH, loss-of-function etfqo-1, d2hgdh-2 and ivdh-1 mutants displayed compromised respiration rates and were more sensitive to drought. Our results demonstrated that an operational ETF/ETFQO pathway is associated with plants' ability to withstand drought and to recover growth once water becomes replete. Drought-induced metabolic reprogramming resulted in an increase in tricarboxylic acid (TCA) cycle intermediates and total amino acid levels, as well as decreases in protein, starch and nitrate contents. The enhanced levels of the branched-chain amino acids in loss-of-function mutants appear to be related to their increased utilization as substrates for the TCA cycle under water stress. Our results thus show that mitochondrial metabolism is highly active during drought stress responses and provide support for a role of alternative respiratory pathways within this response.

Branched-chain amino acids

Tricarboxylic acid cycle

ETF/ETFQO pathway

Drought

Metabolomics

Respiration

Författare

Marcel V. Pires

Universidade Federal de Vicosa

Max Planck-institutet

Adilson A. Pereira Júnior

Max Planck-institutet

David B. Medeiros

Universidade Federal de Vicosa

Danilo M. Daloso

Max Planck-institutet

Universidade Federal de Vicosa

Phuong Anh Pham

Max Planck-institutet

Kallyne A. Barros

Universidade Federal de Vicosa

Martin Engqvist

Chalmers, Biologi och bioteknik, Systembiologi

A. Florian

Max Planck-institutet

Ina Krahnert

Max Planck-institutet

V. G. Maurino

Heinrich Heine Universität Düsseldorf

Wagner L. Araújo

Universidade Federal de Vicosa

A. R. Fernie

Max Planck-institutet

Plant, Cell and Environment

0140-7791 (ISSN) 1365-3040 (eISSN)

Vol. 39 6 1304-1319

Ämneskategorier

Bioinformatik och systembiologi

DOI

10.1111/pce.12682

Mer information

Senast uppdaterat

2018-02-21