Studying Z-DNA and B-to Z-DNA transitions using a cytosine analogue FRET-pair
Artikel i vetenskaplig tidskrift, 2016

Herein, we report on the use of a tricyclic cytosine FRET pair, incorporated into DNA with different base pair separations, to study Z-DNA and B-Z DNA junctions. With its position inside the DNA structure, the FRET pair responds to a B- to Z-DNA transition with a distinct change in FRET efficiency for each donor/acceptor configuration allowing reliable structural probing. Moreover, we show how fluorescence spectroscopy and our cytosine analogues can be used to determine rate constants for the B- to Z-DNA transition mechanism. The modified cytosines have little influence on the transition and the FRET pair is thus an easily implemented and virtually non-perturbing fluorescence tool to study Z-DNA. This nucleobase analogue FRET pair represents a valuable addition to the limited number of fluorescence methods available to study Z-DNA and we suggest it will facilitate, for example, deciphering the B- to Z-DNA transition mechanism and investigating the interaction of DNA with Z-DNA binding proteins.

Författare

Blaise Dumat

Chalmers, Kemi och kemiteknik, Kemi och biokemi, Fysikalisk kemi

Anders Foller Füchtbauer

Chalmers, Kemi och kemiteknik, Kemi och biokemi, Fysikalisk kemi

Marcus Wilhelmsson

Chalmers, Kemi och kemiteknik, Kemi och biokemi, Fysikalisk kemi

Nucleic Acids Research

0305-1048 (ISSN) 1362-4962 (eISSN)

Vol. 44 e101

Styrkeområden

Nanovetenskap och nanoteknik

Livsvetenskaper och teknik

Ämneskategorier

Fysikalisk kemi

Biokemi och molekylärbiologi

Analytisk kemi

Biofysik

Fundament

Grundläggande vetenskaper

DOI

10.1093/nar/gkw114

PubMed

26896804